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OBJECTIVE To study the changes in the matrix metalloproteinases-2 and 9(MMP2,MMP9)induced by 60Coγ-ray external irradiation of human prostate cancer PC-3 cells. METHODS Human prostate cancer PC-3 cells were irradiated with different doses of 60Coγ-rays.Cel migration and invasiveness were evalu- ated and the expression of MMP2,and MMP9 was investigated by RT-PCR, Western blotting and flow cytometry(FCM). RESULTS Irradiation enchances invasive protential at the doses of 1,3 and 5 Gy,whereas it significantly inhibits cell migration. CONCLUSION The different doses of 60Coγ-ray external irradiation for prostate cancer may have different effects through the changes of MMP2, and MMP9 expression.
OBJECTIVE To study the changes in the matrix metalloproteinases-2 and 9 (MMP2, MMP9) induced by 60Coγ-ray external irradiation of human prostate cancer PC-3 cells. Methods Human prostate cancer PC- rays.Cel migration and invasiveness were evaluated- ated and the expression of MMP2, and MMP9 was investigated by RT-PCR, Western blotting and flow cytometry (FCM). RESULTS Irradiation enchances invasive at the doses of 1, 3 and 5 Gy, it has significantly inhibits cell migration. CONCLUSION The different doses of 60Coγ-ray external irradiation for prostate cancer may have different effects through the changes of MMP2, and MMP9 expression.