论文部分内容阅读
探讨苯、甲醛致体外培养细胞的中国仓鼠肺成纤维细胞DNA损伤的联合作用方式。用正交设计方案,用甲醛(0,0.31975,0.6395μg/ml),苯(0,0.392,0.784mg/ml),甲醛+苯(0.31975μg/ml+0.392mg/ml,0.31795μg/ml+0.784mg/ml,0.6395μg/ml+0.392mg/ml,0.6395μg/ml+0.784mg/ml)对中国仓鼠肺成纤维细胞染毒24h,然后通过细胞凋亡试验、单细胞凝胶电泳和DNA交联试验检测苯、甲醛致细胞DNA损伤的联合作用方式。细胞凋亡试验、单细胞凝胶电泳和DNA交联试验结果经正交实验方差分析,苯与甲醛单独或联合作用于CHL细胞,在剂量设置范围内,随剂量增加细胞凋亡率增加,细胞DNA交联率增加;苯单独作用于CHL细胞,在剂量设置范围内,随剂量增加CHL细胞DNA断裂增加,甲醛单独作用于CHL细胞,在剂量设置范围内,低剂量组细胞DNA断裂高于高剂量组,且与溶剂对照组比较,差异均有统计学意义。此外,结果显示甲醛、苯两因素具交互作用(P<0.05)。苯、甲醛均可以引起体外培养细胞的DNA损伤,而且这种损伤作用具有剂量-效应关系。当苯与甲醛共同作用于体外培养细胞时,其引起的DNA损伤作用在一定浓度范围内具有协同作用。
To investigate the combined effect of benzene and formaldehyde on DNA damage of Chinese hamster lung fibroblasts cultured in vitro. (0,0.31975,0.6395μg / ml), benzene (0,0.392,0.784mg / ml), formaldehyde + benzene (0.31975μg / ml + 0.392mg / ml, 0.31795μg / ml + Chinese hamster lung fibroblasts were exposed to 0.784mg / ml, 0.6395μg / ml + 0.392mg / ml, 0.6395μg / ml + 0.784mg / ml for 24 hours and then tested by apoptosis test, single cell gel electrophoresis and DNA Crosslinking test detects benzene, formaldehyde induced cell DNA damage in a joint mode of action. Cell apoptosis test, single cell gel electrophoresis and DNA cross-linking test results by orthogonal test analysis of variance, benzene and formaldehyde alone or in combination on the role of CHL cells in the dose setting range, with increasing dose of apoptosis rate, The DNA cross-linking rate increased; benzene alone treated CHL cells in a dose setting range, increased DNA fragmentation with increasing doses of CHL cells, formaldehyde alone acting on CHL cells in the dose setting range, low-dose group cells DNA fragmentation was higher than high Dose group, and compared with the solvent control group, the differences were statistically significant. In addition, the results showed that formaldehyde and benzene interact with each other (P <0.05). Benzene and formaldehyde can cause DNA damage of cultured cells in vitro, and this damage has a dose-effect relationship. When benzene and formaldehyde co-act in vitro cultured cells, the DNA damage caused by its role in a certain concentration range has a synergistic effect.