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螺旋粉虱Aleurodicus dispersus Russell是一种重要的农林害虫。本研究分别利用次级内共生菌Cardinium和Arsenophonus的16S rDNA和Wolbachia wsp基因对海南省16地区的螺旋粉虱的3种次级内共生菌Cardinium,Arsenophonus和Wolbachia感染情况及相关基因序列进行了测定和分析。3种次级内共生菌Cardinium,Arsenophonus和Wolbachia检测结果表明,Cardinium和Arsenophonus均可感染海南地区的螺旋粉虱,其中乐东、陵水和澄迈3个地区所有寄主上的螺旋粉虱的Arsenophonus感染率为100%,三亚、琼中和临高部分寄主上的螺旋粉虱的Arsenophonus感染率为66.7%,而儋州、五指山和万宁3个地区的螺旋粉虱未发现被Arsenophonus感染;三亚的番石榴上的螺旋粉虱的Cardinium感染率为100%,琼海白沙的印度紫檀上螺旋粉虱的Cardinium感染率为100%,其他寄主上的感染率均小于66.7%;在所检测的43个螺旋粉虱种群中,40和31个种群中分别检测出有Arsenophonus和Cardinium感染,种群感染率分别为93.0%和72.1%;在所有检测的个体中,120个个体中有105个感染Arsenophonus,93个个体中有70个感染Cardinium,个体感染率分别为87.5%和75.3%;在检测的所有样本中,只有三亚印度紫檀上的螺旋粉虱检测到Wolbachia,种群感染率为2.3%,个体感染率仅为0.8%。这些检测结果表明,海南地区螺旋粉虱次级内共生菌Arsenophonus的感染率高于Cardinium的感染率,Wolbachia的感染率极低。序列分析表明,海南不同螺旋粉虱种群的Cardinium的16S rDNA序列一致,而且与烟粉虱的Cardinium 16S rDNA序列一致性很高,为97.6%;不同螺旋粉虱种群的Arsenophonus的16SrDNA序列也完全一致,其与西班牙加那利群岛螺旋粉虱的Arsenophonus的16S rDNA序列一致性较高,为85.1%。此外,Wolbachiawsp基因序列分析表明,海南螺旋粉虱的Wolbachia为B组,这是国内螺旋粉虱感染Wolbachia的首次报道。
Aleurodicus dispersus Russell is an important agroforestry insect. In this study, three sub-endosygobacterial Cardinium, Arsenophonus and Wolbachia infections and their related gene sequences were determined by using 16S rDNA and Wolbachia wsp genes of Cardinium and Arsenophonus, respectively. And analysis. The results of Cardinium, Arsenophonus and Wolbachia showed that both Cardinium and Arsenophonus could infect the whiteflies in Hainan Province. Among them, Arsenophonus was found on all hosts in Ledong, Lingshui and Chengmai, The infection rate was 100%. The infection rate of Arsenophonus was 66.7% on the whiteflies in Sanya, Qiongzhong and Progeny, and the Arsenophonus infection was not found in the three areas of Danzhou, Wuzhishan and Wanning. Of the Cardiotoxicity of B. tabaci on the guava was 100%. The Cardinium infection of the B. tabaci on Qionghai Baisha was 100%, while the infection rate on other hosts was less than 66.7%. In the detected 43 Arsenophonus and Cardinium infections were detected in 40 and 31 populations of the B. tabaci populations, with 93.0% and 72.1% of the population infections, respectively; 105 of 120 individuals were infected with Arsenophonus in all tested individuals, 70 of 93 individuals were infected with Cardinium, and the individual infection rates were 87.5% and 75.3%, respectively. Of all the samples tested, only Wolbachia was detected by B. tabaci on Sanya Indian red sandal and the infection rate was 2.3% Infection rate was only 0.8%. The results of these tests showed that the infection rate of secondary endosymbionts Arsenophonus in Hainan Province was higher than that of Cardinium, and the infection rate of Wolbachia was very low. Sequence analysis showed that the 16S rDNA sequences of Cardinium in different populations of B. tabaci were the same in Hainan, and the sequence identities with Cardinium 16S rDNA of B. tabaci were 97.6%. The 16S rDNA sequences of Arsenophonus from different populations of B. tabaci were also consistent , Which is highly consistent with the 16S rDNA sequence of Arsenophonus of the Spanish whitefly, Canary Islands, at 85.1%. In addition, Wolbachiawsp gene sequence analysis showed that Wolbachia of B. tabaci was a group B, which was the first report of Wolbachia infection by B. spiralis in China.