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目的探讨S波段高功率微波(S band high power microwave,S-HPM)辐射对大鼠睾丸Toll样受体(Toll-like receptor,TLR)表达的影响。方法 60只雄性Wistar大鼠经0 m W/cm2、30 m W/cm2和100 m W/cm2的S-HPM辐射20 min,通过TUNEL方法检测辐射后6 h、3 d、7 d和14d睾丸生精细胞的凋亡。采用Real-time PCR和Western blotting检测辐射后睾丸组织TLR2、TLR3、TLR4和TLR5的mRNA和蛋白水平的表达变化。结果经30 m W/cm2和100 m W/cm2的S-HPM辐射后,生精细胞凋亡率随时间延长而增加,100 m W/cm2组重于30 m W/cm2组(P<0.05);S-HPM辐射后6 h、3 d和7 d可见TLR2-5 mRNA表达不同程度的增加(P<0.05,P<0.01);辐射后7 d可见TLR2-5蛋白表达的增加,100 m W/cm2与30 m W/cm2组相比无显著差异。结论 S-HPM辐射可致睾丸生精组织凋亡,TLR2-5的表达上调,参与了微波辐射致生精细胞凋亡的病理生理过程。
Objective To investigate the effect of S-band high power microwave (S-HPM) radiation on the expression of Toll-like receptor (TLR) in rat testes. Methods Sixty male Wistar rats were irradiated with S-HPM at 0 mW / cm 2, 30 mW / cm 2 and 100 mW / cm 2 for 20 min. TUNEL method was used to detect testis at 6 h, 3 d, 7 d and 14 d Spermatogenic cell apoptosis. The mRNA and protein levels of TLR2, TLR3, TLR4 and TLR5 were detected by Real-time PCR and Western blotting. Results The apoptotic rate of spermatogenic cells increased with the increase of time after S-HPM irradiation at 30 mW / cm2 and 100 mW / cm2, and the apoptosis rate at 100 mW / cm2 was higher than that at 30 mW / cm2 (P <0.05 (P <0.05, P <0.01). The expression of TLR2-5 protein increased at 6 h, 3 d and 7 d after S-HPM irradiation and the expression of TLR2-5 protein increased by 100 m There was no significant difference between W / cm2 and 30 mW / cm2. Conclusion S-HPM radiation can induce apoptosis of spermatogenic cells in testes, and the expression of TLR2-5 is up-regulated, which is involved in the pathophysiological process of apoptosis of spermatogenic cells induced by microwave radiation.