论文部分内容阅读
高速逆流层析与热喷式质谱联用(HSCCC/MS)法是一种新近发展起来的、具有适用范围广和分辨率高等特点的分析方法,它不需要对 HSCCC 流出液做任何分离就可用于鉴定,而且适用于分析非挥发性或热不稳定性物质。两相溶剂系统为正己烷:乙醇:水=6:5:1(v/v),分析型高速逆流色谱仪配有一个缠绕多圈 PTFE(id 0.85mm)的柱,sd 5cm,转速为2000rpm。柱子先填满固定相(上相),然后在转速1500rpm 下,泵入流动相(下相),当流出液澄明后注入样品液。
High-speed countercurrent chromatography coupled with thermal spray mass spectrometry (HSCCC / MS) is a newly developed analytical method with a wide range of applications and high resolution that can be used without any separation of the HSCCC effluent In the identification, but also for the analysis of non-volatile or heat-labile substances. The two-phase solvent system was n-hexane: ethanol: water = 6: 5: 1 (v / v) and the analytical high speed countercurrent chromatograph was equipped with a column of multi-turn PTFE (id 0.85 mm) . The column was filled with the stationary phase (upper phase) first, and then pumped into the mobile phase (lower phase) at a speed of 1500 rpm, and the sample liquid was injected when the effluent was clear.