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目的:以异槲皮素为转化对象对肠内菌体外转化模型的转化稳定性进行考察。方法:按照先前建立的《人肠内细菌生物转化模型和标准操作规程》,采用人源不同及人源相同但培养代数不同的肠内菌建立体外转化模型,对异槲皮素进行生物转化。结果:在24 h内,采用不同人源的肠内菌建立的转化模型均可将异槲皮素转化为槲皮素,但是转化所需的时间存在差异;而随着培养代数的增加,肠内菌表现出转化效率增强的趋势。结论:本研究证明,按照《标准操作规程》建立的肠内菌体外转化模型比较稳定,所得实验结果具有重现性,可用于中药化学成分的肠内菌转化研究。
OBJECTIVE: To investigate the stability of transformation of enterobacteriaceae transformed by isoquercitrin in vitro. Methods: In accordance with the previously established “human intestinal bacterial biotransformation model and standard operating procedures”, using different human sources and the same source but different albumen culture albumen model in vitro transformation of isoquercetin biotransformation. RESULTS: Isoquercitrin was converted to quercetin using a transformation model established by different human enteric bacteria within 24 h, but the time required for transformation was different. However, with the increase of culture algebra, The internal bacteria showed an increasing trend of transformation efficiency. Conclusion: This study demonstrates that the model of in vitro transformation of enterobacteria established in accordance with the “Standard Operating Procedures” is relatively stable and the results obtained are reproducible and can be used for the study of the transformation of enterobacteria in the chemical constituents of traditional Chinese medicine.