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【目的】在无机三态氮(氨氮、亚硝氮和硝氮)共存的模拟海水水体中,阐明有机碳尤其是海藻寡糖对海洋着色菌(Marichromatium gracile)YL28生长及去除无机三态氮的影响规律。【方法】采用次溴酸钠氧化法、N-(1-萘基)-乙二胺分光光度法和紫外分光光度法分别测定水体中氨氮、亚硝氮和硝氮的含量,菌体生物量采用比浊法测定。【结果】在光照厌氧环境中,小分子有机酸盐(乙酸钠、丙酮酸钠、琥珀酸钠和柠檬酸钠)是YL28生长和去除无机三态氮的良好有机碳,亚硝氮、硝氮和氨氮去除率分别达到97.92%、99.98%、73.23%-87.15%。单糖(葡萄糖和果糖)、双糖(麦芽糖和蔗糖)和寡糖(壳寡糖和海藻寡糖)是YL28可利用的有机碳,亚硝氮和硝氮去除率分别达到99%和87%以上,氨氮去除率在44.82%-54.53%之间。多糖(β-环糊精、淀粉、黄原胶、琼脂粉、海藻酸钠和卡拉胶)不是菌体利用和去除无机三态氮的有机碳。酵母提取物可作为菌体生长、去除硝氮和亚硝氮的良好有机碳,但严重抑制氨氮去除。海藻酸钠、β-环糊精和卡拉胶分别与乙酸钠共存时,YL28生长和对无机三态氮去除能力与乙酸钠为唯一有机碳的水平相当。乙酸钠体系中添加海藻寡糖,YL28生长速率、最大生物量以及氨氮的去除速率和最大去除率均升高,添加酵母提取物时,生长速率和最大生物量升高,但氨氮去除速率和最大去除率降低。黑暗厌氧环境下,以乙酸钠和氨氮为唯一有机碳和氮源时,YL28不生长,但在无机三态氮共存时,则能良好生长并去除无机三态氮。【结论】在无机三态氮共存海水体系和厌氧条件下,无论是光照还是黑暗环境,YL28均能良好地生长和去除无机三态氮,小分子有机酸盐(乙酸钠、丙酮酸钠、琥珀酸钠、柠檬酸钠)是其良好的有机碳,相对而言,乙酸钠和丙酮酸钠更好。海藻寡糖与乙酸钠复合可提高菌体生长和脱氮能力。本研究为研制开发高效脱氮微生物制剂及其合理性应用提供了指导。
【Objective】 The effects of organic carbon, especially seaweed oligosaccharides, on the growth and removal of inorganic trichomes of nitrogen in Marichromatium gracile YL28 were studied in simulated seawater with coexistence of inorganic trinitrogen (ammonia nitrogen, nitrite nitrogen and nitrate nitrogen) Affect the law. 【Method】 The contents of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen in water were determined by the method of sodium hypobromite oxidation, N- (1-naphthyl) -ethylenediamine spectrophotometry and ultraviolet spectrophotometry, Turbidimetric determination. 【Result】 The results showed that small organic acids (sodium acetate, sodium pyruvate, sodium succinate and sodium citrate) were good organic carbon, nitrite nitrogen and nitrate for growth and removal of inorganic tristate nitrogen in light and anaerobic environment. Nitrogen and ammonia nitrogen removal rates were 97.92%, 99.98%, 73.23% -87.15%. Monosaccharides (glucose and fructose), disaccharides (maltose and sucrose) and oligosaccharides (chitooligosaccharides and seaweed oligosaccharides) are available organic carbon YL28, nitrite and nitrate nitrogen removal rates were 99% and 87% Above, ammonia removal efficiency of 44.82% -54.53% between. Polysaccharides (β-cyclodextrin, starch, xanthan gum, agar powder, sodium alginate and carrageenan) are not organic carbons that utilize and remove inorganic tristate nitrogen in the cell body. Yeast extract can be used as cell growth, removal of nitrogen and nitrite nitrogen good organic carbon, but seriously inhibited ammonia nitrogen removal. When sodium alginate, β-cyclodextrin and carrageenan coexist with sodium acetate, respectively, the ability of YL28 growth and removal of inorganic tristate nitrogen is comparable to that of sodium acetate as sole organic carbon. The addition of alginate oligosaccharides to sodium acetate system increased the growth rate, maximum biomass, ammonia nitrogen removal rate and maximum removal rate of YL28. When yeast extract was added, the growth rate and maximum biomass increased, but the maximum removal rate of ammonia nitrogen Removal rate decreased. In dark anaerobic environment, YL28 did not grow when sodium acetate and ammonia nitrogen were the only organic carbon and nitrogen sources, but could grow well and remove inorganic trinitrogen when inorganic tri-state nitrogen coexisted. 【Conclusion】 YL28 can grow and remove inorganic trinitrogen, small organic acid salts (sodium acetate, sodium pyruvate, sodium pyrophosphate, Sodium succinate, sodium citrate) are good organic carbons and, in contrast, sodium acetate and sodium pyruvate are better. Seaweed oligosaccharides and sodium acetate compound can improve cell growth and denitrification ability. This study provides guidance for the research and development of high-efficiency denitrifying microbial agents and their rational application.