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9-羰基-10,11-去氢泽兰酮为紫茎泽兰(Eupatorium adenophorum)的主要致肝脏毒性成分及杀虫的生物活性成分。从紫茎泽兰叶片中分离提纯得到9-羰基-10,11-去氢泽兰酮(Euptox A)标准品,建立了高效液相色谱法测定紫茎泽兰中Euptox A含量的分析方法。采用C18反相色谱柱,柱温30°C,以甲醇-水(60:40,v/v)为流动相、流速为0.8 mL.min–1、检测波长为255 nm进行测定。Euptox A在紫茎泽兰中的添加回收率为97.3%–103.7%,检测限为0.4μg.g–1。利用建立的方法测定Euptox A在紫茎泽兰体内分布与积累的动态变化规律。结果表明,Euptox A主要分布在紫茎泽兰的叶片中,且在营养生长期积累量高,生殖生长期积累量低。该方法快速、简捷,可用于紫茎泽兰原料及其产品中Euptox A成分的测定。
9-carbonyl-10,11-dehydrozemalanone is the major hepatic toxic component and insecticidal bioactive component of Eupatorium adenophorum. The standard of 9-carbonyl-10,11-dehydrozelecone (Euptox A) was isolated and purified from the leaves of Eupatorium adenophorum, and the analytical method for the determination of Euptox A in Eupatorium adenophorum by HPLC was established. The mobile phase was methanol-water (60:40, v / v) at a flow rate of 0.8 mL.min-1 and the detection wavelength was set at 255 nm, using a C18 reversed-phase column with a column temperature of 30 ° C. The recoveries of Euptox A in Eupatorium adenophorum were 97.3% -103.7% with the detection limit of 0.4μg.g-1. The established method was used to determine the dynamic changes of Euptox A distribution and accumulation in Eupatorium adenophorum. The results showed that Euptox A mainly distributed in the leaves of Eupatorium adenophorum, with high accumulation during vegetative growth period and low accumulation during reproductive growth period. The method is rapid and simple and can be used for the determination of Euptox A in raw materials of Eupatorium adenophorum and its products.