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对捕食性节肢动物的捕食作用进行评价是害虫生物防治研究的一个重要内容。本文利用酶联免疫吸附试验(ELISA)定量评价了食虫沟瘤蛛对稻飞虱的捕食作用。结果表明,方法特异性完全符合试验要求;食虫沟瘤蛛在捕食3头3-5龄白背飞虱或褐飞虱若虫后的检出期分别为96小时和120小时;在早稻田中,捕食性天敌对白背飞虱和褐飞虱的阳性反应率分别为19.05%-47.34%和19.05%-66.67%,在飞虱密度较低时,捕食性天敌仍表现出较高的阳性率;捕食量随飞虱密度的增加而增加,但捕食率下降。捕食性节肢动物是调节褐飞虱种群动态的重要因子之一。
Predation of predatory arthropod evaluation of pest biological control is an important part of the study. In this paper, enzyme-linked immunosorbent assay (ELISA) was used to quantitatively evaluate the predation of Botryococcus embossed on planthoppers. The results showed that the specificity of the method met the requirements of the experiment completely. The detection period of carnivores was about 96 hours and 120 hours respectively after preying on 3-instar 3-5-year-old white-backed planthopper or brown planthopper nymphs. In the early rice field, The positive rates of natural enemies to whitebacked planthopper and brown planthopper were 19.05% -47.34% and 19.05% -66.67%, respectively, and the predatory natural enemies showed higher The positive rate increased with the increase of planthopper density, but the predation rate decreased. Predatory arthropods are one of the important factors that regulate the population dynamics of BPH.