从 5例手术切除宫颈癌标本中取材进行体外培养 ,并建成一个新的连续细胞系 ,命名为湖南宫颈癌上皮细胞系 1号 (HCE1)。该细胞已在体外生存 18个多月 ,传 80多代。其生物学特性显示 :①该细胞系接种至裸鼠后可致瘤 ,其移植瘤细胞形态与原实体瘤相似 ;②电镜下可观察到细胞表面有丰富的微绒毛及核糖体 ;③检测第 2 5代细胞生长曲线 ,其倍增时间为 45 8h ;④计数 6个平皿软琼脂集落形成率平均值为 14 8% ;⑤染色体核型分析为非整倍体 ,众数为 85条 Five cases of cervical cancer specimens were selected for in vitro culture and a new continuous cell line was constructed and named as HCE1. The cells have survived in vitro for more than 18 months and passed over 80 generations. The biological characteristics of the cells showed that: ① The tumor cells were inoculated into nude mice and the morphology of the transplanted tumor cells was similar to the original solid tumor; ② The microvilli and ribosomes were observed on the cell surface under electron microscope; 2 5 generation of cell growth curve, the doubling time was 45 8h; ④ count 6 plate average soft agar colonization rate was 14 8%; ⑤ chromosome karyotype analysis of aneuploidy, the mode is 85