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为了更好地研究植物在磷素吸收过程中的分子机制以及生物化学过程的变化,将水稻中分离得到的一个磷转运蛋白基因(OsPT6)用于互补实验。互补实验结果表明,OsPT6能够与缺失磷转运功能的酵母突变体实现互补,并在低磷条件下促进酵母突变体对磷的吸收。进一步分析表明OsPT6属于水稻Pht1家族运输蛋白基因,所编码的蛋白对磷酸盐(Pi)的吸收Km值为96μmol/L,属于高亲和的磷转运蛋白。不同的酵母转化子对不同pH环境的响应实验显示,OsPT6是一个与质子相偶联的磷运输蛋白,其吸收磷素的最佳pH为6.0。对OsPT6在人的胚胎肾细胞(HEK293)中的表达分析表明,该基因能够编码蛋白并定位于细胞膜,证明OsPT6的功能与酵母磷转运子PHO84相似,是一个定位于细胞膜上的具有吸收转运磷素作用的运输蛋白。
In order to better understand the molecular mechanism of plant uptake during phosphorus uptake and the changes of biochemical processes, a phosphate transporter gene (OsPT6) isolated from rice was used for complementation experiments. The results of the complementation experiments showed that OsPT6 can complement the yeast mutant lacking phosphorus transport function and promote the phosphorus uptake by yeast mutant under low phosphorus condition. Further analysis showed that OsPT6 belongs to the Pht1 family transport protein gene of rice, and the encoded protein has a Km value of 96 μmol / L for phosphate (Pi), which belongs to a high-affinity phosphate transporter. Different yeast transformants in response to different pH environment experiments showed that OsPT6 is a proton-coupled phosphorus transport protein, the optimal pH of phosphorus uptake of 6.0. The analysis of the expression of OsPT6 in human embryonic kidney cells (HEK293) showed that this gene encodes the protein and locates in the cell membrane. OsPT6 is proved to be similar to the yeast phosphate transporter PHO84. OsPT6 is a membrane- The role of transport protein.