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目的研究胆管癌抗原肽对转染全长野生型p53的树突状细胞(wtp53DC)免疫功能的影响。方法先将全长野生型p53导入脂质体内并转染小鼠骨髓来源的DC,然后用胆管癌抗原肽修饰wtp53DC,检测这种树突状细胞的抗原提呈功能。结果抗原肽修饰的wtp53DC和单纯DC的上清3种细胞因子含量明显增加,分别为(545.2±12.1)ng/L,(511.1±13.3)ng/L,(537.1±11.1)ng/L(P<0.05);wtp53DC刺激小鼠脾脏T细胞增殖水平明显高于对照组(P<0.01);该细胞高表达B7-1、B7-2、MHC-Ⅰ、MHC-Ⅱ(P<0.05);能够特异性地杀伤胆管癌细胞,杀伤率81.6%。结论全长野生型p53基因转染+胆管癌抗原肽联合修饰树突状细胞能诱导小鼠细胞毒性T淋巴细胞的特异性。
Objective To study the effect of cholangiocarcinoma antigen peptide on immune function of dendritic cells (wtp53DC) transfected with full-length wild-type p53. Methods The full-length wild type p53 was first introduced into liposomes and transfected into bone marrow-derived DCs of mice. The dendritic cells were then modified with wtp53 DC to detect antigen presenting function. Results The contents of three cytokines in wtp53 DC and DC supernatants were significantly increased (541.2 ± 12.1 ng / L, 511.1 ± 13.3 ng / L and 537.1 ± 11.1 ng / L, P <0.05). The proliferation of spleen T cells in wtp53DC group was significantly higher than that in control group (P <0.01). The cells were highly expressed B7-1, B7-2, MHC-I and MHC-Ⅱ Specific killing of cholangiocarcinoma cells, the killing rate of 81.6%. Conclusion Full-length wild-type p53 gene transfection combined with cholangiocarcinoma antigen peptide modified dendritic cells can induce the specificity of mouse cytotoxic T lymphocytes.