[Objective]To research the preparation technology of ursolic acid in HERBA VERBENAE,and to establish its content detection method.[Methods]Ursolic acid was extracted and isolated from HERBA VERBENAE by ethanol extraction and agglutination separation.And content detection was established by the method of HPLC.[Results]After the extraction and isolation,the sample was further identified by spectroscopy,and was verified to be ursolic acid.The purity of ursolic acid reached 99.8%.When detecting its content by HPLC,ursolic acid showed good linear relationship within the range of 4.6-23.0μg/mL(r=0.999 9);and the average recovery rate was 99.82%(RSD was0.75%).[Conclusions]This preparation technology was advanced,rational,practical,feasible,and could be applied in the industrialized production.Detecting the content of ursolic acid by HPLC method was accurate and reliable. [Objective] To research the preparation technology of ursolic acid in HERBA VERBENAE, and to establish its content detection method. [Methods] Ursolic acid was extracted and isolated from HERBA VERBENAE by ethanol extraction and agglutination separation. Ind content detection was established by the method of HPLC. [Results] After the extraction and isolation, the sample was further identified by spectroscopy, and was verified to be ursolic acid. The purity of ursolic acid reached 99.8% .When detecting its content by HPLC, ursolic acid showed good linear relationship The average recovery rate was 99.82% (RSD was 0.75%). [Conclusions] This preparation technology was advanced, rational, practical, feasible, and could be applied in the industrialized production. Detection of the content of ursolic acid by HPLC method was accurate and reliable.