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为建立一个高效的大豆再生体系用于大豆的遗传转化,选用3个东北主栽品种‘黑农35’、‘黑农41’和‘黑农58’的子叶节和胚尖作为外植体,分别建立了3个品种的子叶节和胚尖再生体系,并研究了6-BA对大豆再生的影响。结果表明,‘黑农41’子叶节最适芽诱导培养基为MSB5+1.0mg·L-16-BA+0.2mg·L-1IBA,胚尖最适芽诱导培养基为MSB5+0.2mg·L-16-BA+0.2mg·L-1IBA。‘黑农41’再生体系在出芽率、出芽数和芽伸长数上均远高于‘黑农35’和‘黑农58’,是一个优秀的大豆转基因受体材料。
In order to establish an efficient soybean regeneration system for the genetic transformation of soybean, three cotyledon nodes and embryo tips of northeast main cultivars ’Heinong 35’, ’Heinong 41’ and ’Heinong 58’ were selected as explants, The cotyledonary node and embryo regeneration system of three varieties were established, and the effect of 6-BA on soybean regeneration was studied. The results showed that the optimum culture medium of ’Heinong 41’ was cotyledon MSB5 + 1.0 mg · L-16-BA + 0.2 mg · L-1 IBA, and the embryogenic tip induction medium was MSB5 + 0.2 mg · L -16-BA + 0.2 mg · L-1 IBA. The regeneration system of ’Heinong 41’ was much better than that of ’Heinong 35’ and ’Heinong 58’ in budding rate, sprouting number and bud elongation number, and was an excellent soybean transgene receptor material.