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目的:采用比较蛋白质组学方法研究良性转归葡萄胎和恶性转变葡萄胎中的差异表达蛋白,筛选葡萄胎恶变相关蛋白。方法:分别提取3例良性转归和3例恶性转变完全性葡萄胎组织,进行双向凝胶电泳,质谱鉴定差异蛋白,免疫组化方法分析差异蛋白氯离子通道蛋白1(CLIC1)在葡萄胎和绒毛膜癌中的相对表达量。结果:通过二维电泳分离蛋白和差异比对,共筛选到34个差异蛋白点,经过质谱技术共鉴定出18个差异蛋白。生物信息学分析表明绝大多数差异蛋白参与细胞增殖和细胞存活过程。挑选了一个差异蛋白CLIC1蛋白进行进一步的研究,结果表明绒癌组织中CLIC1明显高于完全性葡萄胎组织,P<0.01。恶性转变葡萄胎组织中CLIC1表达也明显高于良性转归葡萄胎,P<0.01。结论:通过蛋白质组学CLIC1筛选结果表明,CLIC1蛋白可能成为葡萄胎恶性转变的预测指标。
OBJECTIVE: To compare differentially expressed proteins in benign to malignant transitional mole and hydatidiform mole with comparative proteomics and to screen the protein of malignant transformation of hydatidiform mole. Methods: Three cases of benign outcome and three cases of malignant transformation complete hydatidiform mole were extracted, respectively. Two - dimensional gel electrophoresis and mass spectrometry were used to identify the differentially expressed proteins. Immunohistochemistry was used to detect the expression of CLIC1 in hydatidiform mole and Relative expression in choriocarcinoma. Results: A total of 34 differential protein spots were screened by two-dimensional electrophoresis and differential alignment. 18 differential proteins were identified by mass spectrometry. Bioinformatics analysis showed that most of the differential proteins involved in cell proliferation and cell survival process. CLIC1 protein was selected for further study. The results showed that CLIC1 in choriocarcinoma was significantly higher than that in complete hydatidiform mole (P <0.01). CLIC1 expression in malignant transitional mole was also significantly higher than that in benign outcome hydatidiform mole, P <0.01. Conclusion: The CLIC1 proteomics results indicated that CLIC1 protein may be a predictor of the malignant transformation of hydatidiform mole.