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目的:建立鼻窦炎颗粒的质量标准.方法:采用TLC法对黄芩和辛夷进行定性鉴别;采用HPLC法测定黄芩中黄芩苷的含量,色谱柱为Kromasi1 C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-水-磷酸(45:55:0.2),流速为1.0 ml·min-1,检测波长为280 nm,柱温为30℃,进样量为10μl.结果:薄层色谱图斑点清晰,阴性对照无干扰;黄芩苷的线性浓度范围为10.06~100.60μg·ml-1(r=1.0000),平均回收率为96.3%,RSD为0.7%(n=6).结论:该法简单易行,专属性高、重复性好,可用于鼻窦炎颗粒的质量控制.“,”Objective:To establish the quality standard for Bidouyan granule .Methods: Scutellariae radix and Magnoloae flos were identified by TLC.The content of baicalin was determined by HPLC with a Kromasi 1 C18 column (250 mm ×4.6 mm,5 μm). The mobile phase consisted of methanol-water-phosphoric acid (45:55:0.2) at a flow rate of 1.0 ml· min-1, and the injection volume was 10μl.The detection wavelength was 280 nm and the column temperature was 30℃.Results:The spots in TLC were clear without any interference.The linear range of baicalin was 10.06 μg· ml-1-100.60 μg· ml-1 (r =1.0000).The average recovery was 96.3%,and RSD was 0.7%(n=6).Conclusion:The method is simple and specific with good repeatability , which can be used for the quality control of Bidouyan granule .