用含1.345 mg/ml(24 h IC50)蛇床子素的改良TYI-S-33培养基培养蓝氏贾第鞭毛虫滋养体,采用扫描和透射电镜观察蛇床子素作用后24 h虫体超微结构的变化。结果显示,经蛇床子素作用后,鞭毛虫滋养体表面凹凸不平,腹吸盘和中体附近表面有明显的病变,主要表现为腹吸盘表面细胞膜破裂,细胞内容物外溢、稀疏,出现板层样结构,空泡化严重,细胞核畸形,边缘锯齿状,核内染色质边集,吸盘微管部分结构崩解。 The trophozoites of Giardia lamblia were cultured in modified TYI-S-33 medium containing 1.345 mg / ml (IC50) Osthole. Scanning electron microscopy and transmission electron microscopy were used to observe the effects of Osthole on ultrastructure Structural changes. The results showed that after osthole was treated, the surface of trophozoite was uneven, the surface of abdominal suction disk and the middle body had obvious lesions, which were mainly the rupture of cell membrane on the surface of abdominal suction disk, spillover of cell contents, sparse lamella-like appearance The structure, the vacuolization serious, the nuclear deformity, the jagged edge, the chromatin edge of the nucleus, the part of the sucker microtubule disintegration.