Sweet orange genome,the first genome published in Rutaceae,has been sequenced and annotated.Citrus is one of the most important and widely grown fruit crops in the world,with global production ranking first among all the fruit crops.Sweet orange is responsible for about 60%of citrus production.Oranges are mostly polyembryony,male or female sterile and highly heterozygous,these characteristics hindered the genetic and breeding development.A complete genome sequence would provide a valuable molecular framework for genetic breeding and improvement.The genome was sequenced by WGS shotgun strategy.The assembly covers 87%of the estimated ge- Sweet orange genome, the first genome published in Rutaceae, has been sequenced and annotated. Citrus is one of the most important and widely grown fruit crops in the world, with global production first first among all the fruit crops.Sweet orange is responsible for about 60% of citrus production. Orches are mostly polyembryony, male or female sterile and highly heterozygous, these characteristics hindered the genetic and breeding development. A complete genome sequence would provide a valuable molecular framework for genetic breeding and improvement. The genome was sequenced by WGS shotgun strategy. The assembly covers 87% of the estimated ge-