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在单因素试验的基础上,利用响应曲面法对超声辅助提取柚皮中总黄酮的工艺参数进行了优化.以乙醇浓度、超声时间、提取温度为自变量,总黄酮提取率为响应值,采用Box-Be-hnken设计试验方案,利用SAS软件对实验数据进行回归分析,得到黄酮提取率的二次多项式预测模型.在固液比为1∶30的条件下,优化的工艺参数为:乙醇浓度67.0%,超声时间28.0 min,提取温度50.0℃.在此工艺条件下柚皮总黄酮提取率为5.301%.
On the basis of single-factor tests, the parameters of ultrasound-assisted extraction of total flavonoids from pomelo peel were optimized by response surface methodology. The concentration of ethanol, ultrasonic time, and extraction temperature were used as independent variables, and the total flavonoid extraction rate was used as the response value. Box-Be-hnken designed the experimental program and used SAS software to perform regression analysis on experimental data to obtain a quadratic polynomial prediction model for the extraction rate of flavonoids. Under the conditions of a solid-liquid ratio of 1:30, the optimized process parameters were: ethanol concentration 67.0%, ultrasonic time 28.0 min, extraction temperature 50.0°C. The extraction rate of total flavonoids from pomelo peel was 5.301%.