目的　建立乙氧苯柳胺（ Ｅ Ｔ Ｏ Ｆ） 软膏皮肤用药后的血药浓度测定方法。方法　采用高效液相联用电化学检测器。结果　 Ｅ Ｔ Ｏ Ｆ 在６２５ ～１００ μｇ· Ｌ－ １ 呈线型关系，日间差５ ％～６ ％ ，日内差２１ ％ ～６１ ％ ，回收率８６ ％ ～９８ ％ ，最低检测限１２５ ｐｇ· Ｌ－ １ ． Ｅ Ｔ Ｏ Ｆ软膏（１ ｇ 含 Ｅ Ｔ Ｏ Ｆ１５０ ｍｇ） 分别涂在４ 名健康志愿者前臂屈侧４ ｃｍ ×４ ｃｍ 皮肤后，血液中 Ｅ Ｔ Ｏ Ｆ能很快被检出。结论　该方法灵敏度高，重现性好，误差小，能满足 Ｅ Ｔ Ｏ Ｆ临床药代动力学研究需要 Objective To establish a method for determination of plasma concentration of topical oestradiol (E T O F) ointment. Methods High performance liquid chromatography with electrochemical detector. Results E T O F showed a linear relationship at 6  25 ~ 100 μg · L -1, with a difference of 5% -6% during the day, an intraday difference of 21% ~ 61% and a recovery of 86% ~ 98% The lowest detection limit of 125 pg · L- 1. E T O F ointment (1 g with E T O F150 mg) was applied to the skin of 4 cm × 4 cm of forearm flexion in four healthy volunteers, and E T O F in the blood can be quickly detected. Conclusion The method has high sensitivity, good reproducibility, small error and can meet the needs of clinical pharmacokinetics of E T O F