Implant-related infection and early bone integration are the main risk factors of implants for long-term service,to overcome these difficulties,SLA-TiCu surface was prepared by sandblasting and large-grits etching (SLA) treatment on a novel antibacterial titanium-copper alloy (TiCu),which is the most preva-lent surface treatment with micro/submicron hierarchical structures to titanium-based implants.Effects of SLA-TiCu surface on the adhesion,proliferation,apoptosis and differentiation of MC3T3-E1 cells as well as the antibacterial activity against a common orthopedic pathogen (Staphylococcus aureus) were studied.Compared to the following surfaces:sandblasting and large-grits etched pure titanium (SLA-Ti),mechanically ground pure titanium and TiCu alloy (M-Ti and M-TiCu),these results indicated that SLA-TiCu surface obviously enhanced the bone-related gene expressions (alkaline phosphates (ALP),col-lagen type Ⅰ (COL Ⅰ),Runt-related transcription factor 2 (RUN × 2),and osteopontin (OPN)).Moreover,SLA-TiCu surface could maintain a sustainable release of Cu2+ ions and effectively inhibited the viability of bacteria.This study demonstrated that SLA-TiCu surface possessed multifunctional characteristics of improved osteogenic ability and antibacterial activity,making it promising as a novel implant material for hard tissue repairs such as orthopedics and dental implants.