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目的探讨张应力加载下骨髓基质细胞力生长因子(MGF)表达的影响及量效关系。方法骨髓基质细胞原代培养后加力不同时间经形态学观察,ELISA检测各组BMSC培养上清中ALP的浓度,Western blot检测加力后骨髓基质细胞MGF的表达。结果机械拉伸刺激下BMSC的形态学特性逐渐发生改变。加载0h、1h、3h、6h、12h、24h位移为1mm,频率为0.5Hz的机械张应力,均可增强BMSC的ALP活性,其中24h时ALP OD值最高(P<0.05)。Western Blot分析BMSC加力后MGF蛋白的表达,结果为细胞加力后MGF在1h开始有变化,MGF表达水平随加力作用时间延长而增高,组间比较差异有统计学意义(P<0.05)。结论 MGF可能通过刺激骨髓基质细胞ALP的分泌而促进其向成骨方向分化。
Objective To investigate the effect of bone marrow stromal cell growth factor (MGF) expression under tension stress and its dose-response relationship. Methods Primary cultured bone marrow stromal cells (BMMSCs) were cultured with different time and morphological changes. The concentrations of ALP in supernatant of BMSCs were detected by ELISA. The expression of MGF in bone marrow stromal cells was detected by Western blot. Results The morphological characteristics of BMSC gradually changed under the mechanical tension stimulation. The mechanical tensile stress at 0h, 1h, 3h, 6h, 12h, 24h shifted 1mm and frequency 0.5Hz, all of which increased the ALP activity of BMSC. The ALP OD of 24h was the highest (P <0.05). Western Blot analysis of BMSC afterburner MGF protein expression, the results of the cells after the addition of force MGF 1h began to change, MGF expression levels increased with the duration of augmentation, the difference between the two groups was statistically significant (P <0.05) . Conclusion MGF may promote its osteogenic differentiation by stimulating the secretion of ALP in bone marrow stromal cells.