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目的 探讨去卵巢大鼠骨细胞凋亡活性及其相关因素。方法 采用 3′ OH末端DNA原位标记技术观察凋亡细胞活性 ;采用免疫组化SABC法观察bcl 2、Fas、转化生长因子 (TGF) β1的表达情况。结果 去卵巢大鼠成骨细胞的凋亡细胞活性为 (4 0 5± 5 2 ) % ,较假手术组 (2 4 5± 3 0 ) %与去卵巢 +尼尔雌醇 /左炔诺孕酮治疗组 [OVX +N/L组 ,(2 6 4± 2 9) % ]明显增加 ,差异有显著性(P <0 0 1) ;去卵巢大鼠破骨细胞的凋亡细胞活性为 (8 4± 1 2 ) % ,明显低于假手术组 (2 4 0± 2 9) %与OVX +N/L组 (2 6 5± 3 1) % ,差异具有显著性 (P <0 0 1)。3组成骨细胞与破骨细胞内bcl 2阳性表达率均较低 ,差异无显著性 (P值均 >0 0 5 ) ;去卵巢大鼠组成骨细胞内Fas(80 0 % )高于假手术组(4 0 0 % )和OVX +N/L组 (4 0 0 % ) ,而破骨细胞内Fas(2 0 0 % )低于假手术组 (70 0 % )和OVX +N/L组(73 3% ) ,后者差异有显著性 (χ2 =7 94,P <0 0 5 ) ,OVX组成骨和破骨细胞内TGFβ1(分别为 2 0 0 %、0 ) ,均低于其他两组 ,前者差异有极显著意义 (χ2 =13 10 4,P <0 0 1)。结论 去卵巢大鼠骨丢失主要是由于雌激素水平低下引起破骨细胞凋亡减少、成骨细胞凋亡活性增加致骨吸收功能明显增加而超过骨形成所致 ;TGFβ1
Objective To investigate the apoptosis of ovariectomized rats and its related factors. Methods The apoptotic cells were observed by in situ 3 ’OH end labeling. The expression of bcl - 2, Fas and TGF - β1 was observed by immunohistochemical SABC method. Results The apoptosis of osteoblasts in ovariectomized rats was (40 ± 5 2)%, which was significantly higher than that in sham-operated rats (24.5 ± 30%) and ovariectomized + nylestriol / levonorgestrel The activity of apoptotic cells in osteoclasts of OVX + N / L group was significantly higher than that of the control group (P <0.01), and the activity of apoptotic cells in OVX + N / L group was (24.4 ± 29)% 8 4 ± 1 2%, which was significantly lower than that of the sham operation group (24 ± 2 9%) and the OVX + N / L group (2 65 ± 3 1)%, the difference was significant (P 0 01 ). The positive rates of bcl 2 expression in osteoblasts and osteoclasts were lower in 3 groups (P> 0.05), and the level of Fas (80 0%) in osteoblasts of ovariectomized rats was higher than that of sham operation Group (40%) and OVX + N / L group (40%), while the osteoclasts Fas (200%) was lower than the sham operation group (70%) and OVX + N / L group (73.3%), the latter was significantly different (χ2 = 7 94, P <0 05), osteoblasts and osteoclasts TGFβ1 (200%, 0 respectively) Group, the former difference was significant (χ2 = 13 10 4, P <0 0 1). Conclusion The loss of bone in ovariectomized rats is mainly attributed to the decrease of osteoclast apoptosis caused by the low level of estrogen, the increase of osteoblast apoptosis activity and the increase of bone resorption, which is more than that of bone formation. TGFβ1