论文部分内容阅读
[目的]研究枸橼酸喷托维林对肝癌HepG2细胞增殖和凋亡的影响。[方法]采用不同浓度(60、75和90μmol/L)的枸橼酸喷托维林处理HepG2细胞48 h,应用MTT(噻唑蓝)法测定细胞的增殖活性,再检测乳酸脱氢酶(LDH)活性,Hoechst 33258染色法检测细胞的凋亡情况。[结果]枸橼酸喷托维林能以浓度依赖的方式抑制HepG2细胞的增殖。对照组与处理组之间LDH活性比较均无显著性差异(P>0.05)。而75μmol/L枸橼酸喷托维林处理48 h能够显著诱导HepG2细胞凋亡。[结论]枸橼酸喷托维林可显著抑制肝癌HepG2细胞增殖,这与其诱导细胞凋亡途径有关。
[Objective] To study the effect of pentobarbitone citrate on the proliferation and apoptosis of HepG2 cells. [Method] HepG2 cells were treated with different concentrations (60, 75 and 90 μmol / L) of pentoxyverine citrate for 48 h. MTT (thiazolyl tetrazolium) assay was used to determine the proliferative activity of the cells. Lactate dehydrogenase ) Activity, cell apoptosis was detected by Hoechst 33258 staining. [Result] Pentoxyverine citrate could inhibit the proliferation of HepG2 cells in a concentration-dependent manner. There was no significant difference in LDH activity between control group and treatment group (P> 0.05). However, treatment with 75μmol / L pentoxyverine citrate for 48 h significantly induced HepG2 cell apoptosis. [Conclusion] Pentoxyverine citrate can significantly inhibit the proliferation of HepG2 cells, which is related to its induction of apoptosis.