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[目的]克隆中华鳖IGFBP1 mRNA基因,分析基因生物信息,检测基因在胚胎和组织中表达情况。[方法]从中华鳖转录组中发掘IGFPB1 mRNA基因,并分析基因序列物信息;利用RT-PCR技术,从肝脏组织克隆IGFPB1c DNA基因,并检测其在胚胎和几种组织中的表达情况。[结果]从转录组中发掘到中华鳖IGFPB1 mRNA基因,基因长3 410 bp,包含81 bp的5’UCR序列,2 531 bp的3’UCR序列,798 bp开放阅读框,编码266个氨基酸,蛋白质分子量29.1 k Da,预测IGFPB1蛋白有1个α结构;对其构建的氨基酸序列系统进化树与传统形态分类相吻合;并在中华鳖受精卵孵化30d和45d的胚胎组织及300日龄的肝脏、肌肉和肾脏组织均检测到基因的表达。[结论]克隆到中华鳖IGFBP1基因,检测到该基因在30 d后的胚胎组织及300日龄的3种组织存在表达。
[Objective] The aim of the study was to clone IGFBP1 mRNA gene in Chinese soft-shelled turtle, analyze gene bioinformation, and detect the expression of IGFBP1 gene in embryos and tissues. [Method] The mRNA of IGFBP1 gene was found from the transcriptome of Chinese soft-shelled turtle, and the gene sequence information was analyzed. The IGFBP1c DNA gene was cloned from liver tissue by RT-PCR, and its expression in embryos and several tissues was detected. [Result] The IGFBP1 mRNA gene was found from the transcriptome. The gene of IGFBP1 was 3 410 bp in length and contained 81 bp of 5’UCR sequence, 2 531 bp of 3’UCR sequence and 798 bp of open reading frame encoding 266 amino acids. The molecular weight of the protein was 29.1 kDa and predicted that the IGFBP1 protein had one α structure. The phylogenetic tree of the constructed amino acid sequence was consistent with the traditional morphological classification. The embryonic tissues incubated for 30 days and 45 days and the liver of 300 days , Muscle and kidney tissue were detected gene expression. [Conclusion] The IGFBP1 gene was cloned from Chinese soft-shelled turtle, and the expression of this gene was detected in embryonic tissues at 30 days and at 300 days of age.