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目的:探讨潮湿环境因素对正常及脾阳虚大鼠一般情况的影响,为建立内外合邪动物模型做初步尝试。方法:选取40只SD大鼠随机分为对照组、脾阳虚组、外湿组、脾阳虚加湿组,每组10只。脾阳虚组模型制作采用番泻叶灌胃,皮下注射利血平与强迫大鼠游泳交替进行的复合方法。造模后,给予外湿组与脾阳虚加湿组潮湿环境1周,于造模前后观察并记录各组大鼠的体质量、进食量、饮水量、精神活动、皮毛光泽度及大便性状并进行宏观症候评分。结果:造模后,脾阳虚加湿组大鼠体质量显著减轻,较其余3组显著降低(P<0.01,P<0.05)。脾阳虚加湿组大鼠在进食、饮水、精神状态、毛色、大便性状等方面较其余3组发生了一定的变化。宏观症候评分较脾阳虚组显著降低(P<0.01)。结论:潮湿环境因素对正常及脾阳虚大鼠的一般情况都有影响,对脾阳虚大鼠影响更为明显。
Objective: To investigate the influence of humid environmental factors on the general situation of normal and spleen yang deficiency rats, and to make a preliminary attempt to establish the animal model of internal and external evils. Methods: Forty Sprague-Dawley rats were randomly divided into control group, spleen-Yang deficiency group, external wetness group and spleen-Yang deficiency plus humidification group, with 10 rats in each group. Spleen yang deficiency model made using senna gavage, subcutaneous injection of reserpine and forced swimming rats alternately. After modeling, the external wet group and the spleen yang deficiency humidification group were given a humid environment for 1 week. The body weight, food intake, water intake, mental activity, fur glossiness and stool traits of the rats in each group were observed and recorded before and after modeling Macroscopic symptom score. Results: After model establishment, the body weight of rats in model group was significantly lower than that in the other three groups (P <0.01, P <0.05). Compared with the other three groups, rats in spleen yang deficiency and humidification group had certain changes in eating, drinking, mental state, coat color and stool character. The macro-symptom score was significantly lower than that of the spleen-yang deficiency group (P <0.01). Conclusion: The wet environmental factors on normal and spleen yang deficiency rats have an impact on the general situation, the spleen yang deficiency rats more obvious.