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目的:研究安神补心片中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量的高效液相测定方法。方法:色谱柱:Welchrom C18色谱柱(250mm×4.6mm,5μm);流动相:乙腈-水(25∶75);流速:0.9mL/min;检测波长:320nm;柱温:25℃;进样量:10μL。结果:2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在0.019 67~0.196 60μg范围内线性关系良好(r=0.999 9),平均回收率为99.220%,RSD为0.54%(n=6)。结论:该方法操作简单、结果准确、专属性强,可用于该制剂的质量控制。
Objective: To study the determination of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside in Anshen Buxin Pill by HPLC. Method: Column: Welchrom C18 column (250 mm × 4.6 mm, 5 μm); mobile phase: acetonitrile-water (25:75); flow rate: 0.9 mL / min; detection wavelength: 320 nm; Amount: 10 μL. Results: The linearity of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside was good in the range of 0.019 67 to 0.196 60 μg (r = 0.999 9) with an average recovery of 99.220 %, RSD 0.54% (n = 6). Conclusion: The method is simple, accurate and specific, and can be used for the quality control of the preparation.