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目的 研究川芎嗪 (TMP)注射液对兔脊髓缺血损伤治疗作用的剂量效应关系 .方法 2 6只新西兰雄性大白兔 ,随机分成四组 :对照组即 C组 (n=6 ) ,单组缺血再灌注 ;T1 组(n=6 )、T2 组 (n=8)和 T3组 (n=6 ) ,分别在肾下主动脉阻断2 0 min后开放时恒速静脉泵入 TMP注射液 15 ,30和 6 0 mg· kg- 1 .采用肾下主动脉 (IRA)阻断法造成脊髓缺血 (2 0min) /再灌注模型 ,观察再灌注后 4,8,12 ,2 4和 48h神经功能评分 (neurologic deficit score) ,并于 48h处死动物取脊髓(L5 - L7)制标本行病理组织学观察 .结果 用不同剂量TMP治疗的 T1 ,T2 和 T3组在各时间点神经功能评分和再灌注 48h脊髓前角正常神经元数均明显高于 C组 (P<0 .0 1) ,且 T2 组和 T3又明显高于 T1 组 (P<0 .0 5 ) ,而 T2 组和 T3组无显著差异 ,神经功能评分与其对应脊髓前角正常神经元计数之间有显著相关性 (r=0 .776 ,P<0 .0 1) .结论 TMP注射液对脊髓缺血损伤有治疗作用 ,并呈一定的剂量效应关系
Objective To study the dose-response relationship of tetramethylpyrazine (TMP) injection for treatment of spinal cord ischemic injury in rabbits. METHODS: Twenty-six New Zealand male rabbits were randomly divided into four groups: the control group (group C) (n=6). Blood reperfusion; T1 group (n=6), T2 group (n=8), and T3 group (n=6), respectively, TPV injection at a constant rate after 20 minutes of infrarenal aorta blockade. 15, 30, and 60 mg·kg-1. Spinal cord ischemia (20 min)/reperfusion model was performed using the renal infrarenal aorta (IRA) blocking method. Observations were made 4, 8, 12, 24, and 48 h after reperfusion. Neurological deficit scores were performed and the animals were sacrificed at 48h to obtain spinal cord (L5-L7) specimens for histopathological observation. The results were evaluated by neurological function scores at different time points in T1, T2, and T3 groups treated with different doses of TMP. The number of normal neurons in the anterior horn of the spinal cord at 48 h after reperfusion was significantly higher than that in the C group (P<0.01), and T2 and T3 were significantly higher than those in the T1 group (P<0.05), while the T2 and T3 groups. There was no significant difference between the groups, and there was a significant correlation between the neurological function score and the corresponding normal neurons in the anterior horn of the spinal cord (r=0.776, P<0.01). Conclusion TMP injection has no effect on the spinal cord Injury treatment, and in a dose-response relationship