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对象 SD大鼠44只,其中雄鼠14只,雌鼠30只。分为雄、雌、去卵巢雌鼠(OVX)三组。14只雌鼠接受经背部肋弓下切口的双侧卵巢摘除术,康复2周制备成OVX鼠。 方法 水合氯醛(500mg/kg)腹腔注射麻醉动物,按Pxinos等鼠脑定向图谱,将刺激电极和碳纤维工作电极分别置入前脑内侧束(MFB)和纹状体(Str),或腹侧背盖区(VTA)和杏体核(Amy)。给予MFB2.0～2.5mA、200～250次脉冲(P),125Hz,0.2ms波宽的方波直流电刺激,诱发Str的最大多巴胺(DA)释放量;电刺激VTA诱发Amy最大DA释放的刺激参数为1.0mA、20p、60～80Hz、350μs。用快速周期伏安法(FCV)和碳纤维工作电极检测Str或Amy的DA释放量。当在两脑
Forty-four SD rats, including 14 males and 30 females. Divided into male, female and ovariectomized female rats (OVX) three groups. Fourteen females underwent bilateral ovariectomy via an incision in the dorsal rib cage and were reconstituted for 2 weeks as OVX rats. Methods Chloral hydrate (500mg / kg) was intraperitoneally injected into the anesthetized animal. According to the brain orientation map of Pxinos et al., The stimulation electrodes and carbon fiber working electrodes were placed into the medial foramen (MFB) and striatum (Str) Back area (VTA) and apricot body (Amy). The maximal dopamine (DA) release was induced by MFB2.0 ~ 2.5mA, 200 ~ 250 pulses (P), 125Hz and 0.2ms wave width. Strings were stimulated by electrical stimulation of VTA. The parameters are 1.0mA, 20p, 60 ~ 80Hz, 350μs. Rapid release cyclic voltammetry (FCV) and carbon fiber working electrode were used to measure DA release of Str or Amy. When in two brains