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该文采用PCR方法特异性扩增恶性疟原虫海南株 (FCC - 1 HN)SERA基因片段 ,并将该基因片克隆于M 13噬菌体 ,用双脱氧链末端终止法进行测序 ,应用PCGENE软件对该基因序列进行了分析 ,结果显示该基因片段长度为 45 3bp ,A +T G +C为 2 .6∶1,符合恶性疟原虫结构基因的特点。同源性分析显示该基因在不同虫株间高度保守 ,我国FCC - 1 HN虫株SERA与B1、B2、B3(巴西 )株及S16 (塞内加尔 )株仅一个氨基酸不同 ,与FCR3(刚比亚 )、FCBR(哥伦比亚 )、及S14、S15 (塞内加尔 )等其它虫株SERA基因序列完全一致。抗原表位预测分析显示该多肽N端和C端可能有抗原表位存在
In this paper, the SERA gene of Plasmodium falciparum (FCC - 1 HN) was amplified by PCR. The fragment was cloned into M 13 phage and sequenced by dideoxy chain termination method. The gene sequence analysis showed that the gene fragment length of 45 3bp, A + TG + C is 2.6: 1, in line with the characteristics of Plasmodium falciparum structural gene. Homology analysis showed that the gene was highly conserved among different insect strains. The strain SERA of FCC - 1 HN in China was only one amino acid different from that of strain B1, B2, B3 (Brazil) and S16 (Senegal) ), FCBR (Colombia), and S14, S15 (Senegal) and other strains of other strains of SERA gene sequence exactly the same. Epitope predictive analysis showed that there may be epitopes on the N-terminal and C-terminal of the polypeptide