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本文用放射免疫测定法研究强啡肽A-(1-13)对小鼠脊髓ir-SP含量的影响及其与吗啡影响的相互作用,结果表明: 1.皮下注射吗啡(5mg/kg)可明显增加小鼠脊髓ir-SP浓度。 2.脑室注射强啡肽A-(1-13),以剂量依赖的型式增加小鼠脊髓ir-SP浓度,5μg作用不明显,10μg有明显作用,20μg作用更强,40μg作用与20μg基本一致,达到饱和。 3.强啡肽A-(1-13)与吗啡同时注射,在多数情况下,脊髓ir-SP水平与单独给吗啡或强啡肽A-(1-13)时没有统计学显著差别,但强啡肽A-(1-13)一定剂量时(20μg,40μg)与吗啡配合应用可呈现明显的效应抵消现象。
In this paper, radioimmunoassay was used to study the effect of dynorphin A- (1-13) on the content of ir-SP in the spinal cord of mice and its interaction with morphine. The results showed that: 1. Subcutaneous injection of morphine (5mg / kg) Significantly increased the concentration of ir-SP in mouse spinal cord. 2. Intracerebroventricular injection of dynorphin A- (1-13), a dose-dependent increase in the concentration of ir-SP in the spinal cord of mice, 5μg effect is not obvious, 10μg has a significant role, 20μg stronger, 40μg role and 20μg basically the same , Reach saturation. 3. Simultaneous injections of dynorphin A- (1-13) with morphine did not show statistically significant differences in spinal cord ir-SP levels compared with morphine or dynorphin A- (1-13) alone, but in most cases, The dynorphin A- (1-13) at a certain dose (20μg, 40μg) with morphine can be applied with a significant effect of the counterattack phenomenon.