论文部分内容阅读
应用免疫细胞化学PAP法,研究了鸡胚和雏鸡后根神经节细胞内多巴胺递质的表现与胚胎发育过程中的变化,并在细胞培养条件下研究了周围靶组织——皮肤对后根节细胞胚胎发育的影响。鸡后根节细胞内多巴胺的免疫反应最先出现于E_(10),只占后根节细胞的0.8%,E_(18)时达5.6%,出壳后雏鸡为8.6%。在鸡后根节内,多巴胺免疫反应阳性细胞主要为较大的A类细胞,和极少较小的B类细胞。免疫反应阳性的周围神经末梢位于皮肤和交感干内。当取自E_9的鸡胚后根节细胞经7天培养后,部分神经细胞出现多巴胺免疫反应阳性;而取自E_6的鸡胚后根节细胞虽经10天培养后仍为免疫反应阴性;然而,将取自E_6后根节细胞与皮肤组织联合培养10天后,少量神经细胞及轴突出现多巴胺免疫阳性反应。作者认为:鸡后根节多巴胺细胞在胚胎时期的发育有赖于周围靶组织的作用。
Immunocytochemical PAP method was used to study the expression of dopamine neurotransmitters and changes during embryonic development in chick embryo and chick embryo ganglion cells. The effect of surrounding target tissues - Effect of cell embryo development. The immunoreaction of dopamine in the root cells of chickens was the first to occur in E_ (10), accounting for only 0.8% of the cells in the dorsal root ganglion, 5.6% at E 18 and 8.6% after the chicks were hatched. Within the dorsal root ganglion, dopamine-immunoreactive cells are predominantly larger type A cells and rarely smaller B-type cells. Immunoreactive peripheral nerve endings are located within the skin and sympathetic trunk. When cultured from 7 days, some neurons showed dopamine immunoreactivity when E_9-derived cells were cultured in vitro, while the cells from E_6 were still negative after 10 days of culture. However, , Will be taken from E_6 after root ganglion cells and skin tissue culture for 10 days, a small amount of nerve cells and axons appear dopamine-positive immune response. The authors suggest that the development of dopaminergic cells in the chicken hindbrain stage during embryonic development depends on the role of the surrounding target tissue.