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目的观察在超速心室起搏(ventricular overdrive pacing,VOP)预适应延迟保护阶段热休克蛋白70(HSP70)的表达水平。方法新西兰兔24只,随机分为3组,单纯结扎组,起搏组,起搏+放线菌素D组。制作超速起搏预适应和缺血/再灌注的动物模型,检测CK和CK-MB的变化,动态描记再灌注时心电图,免疫组织化染色检测HSP70抗原。结果缺血后起搏组心肌酶的水平在再灌注时均低于单纯结扎组和起搏+放线菌素D组(P<0.01);单纯结扎组中在再灌注过程中共有5只发生心律失常,起搏+放线菌素组也有4只,而起搏组无心律失常发生。起搏组和单纯结扎组之间有显著性差别(P<0.05)。起搏组HSP70的阳性表达的程度明显高于其他两组。结论超速起搏预适应可以模拟缺血预适应,其延迟保护作用可能与HSP70表达增加密切相关。
Objective To observe the expression of heat shock protein 70 (HSP70) during the preconditioning delayed protection phase of ventricular overdrive pacing (VOP). Methods Twenty-four New Zealand rabbits were randomly divided into three groups: simple ligation group, pacing group, paced + dactinomycin group. The models of hyperspeed pacing preconditioning and ischemia / reperfusion were made. The changes of CK and CK-MB were detected. The electrocardiogram was recorded during the dynamic electrocardiographic reperfusion and the HSP70 antigen was detected by immunohistochemistry. Results The levels of myocardial enzymes in the post-ischemic pacing group were significantly lower than those in the simple ligation group and the paced + dactinomycin group (P <0.01), while in the ligation group, 5 Arrhythmia, pacing + actinomycin group also had 4, while no pacing group arrhythmia. There was a significant difference between pacing group and simple ligation group (P <0.05). Pacing group HSP70 positive expression was significantly higher than the other two groups. Conclusion Overexpression of pacing preconditioning can simulate ischemic preconditioning, and its delayed protective effect may be closely related to the increase of HSP70 expression.