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目的:探讨Bag-1和热休克蛋白70(HSP70)在宫颈上皮内瘤变中的关系。方法:采用免疫组织化学S-P法,对64例宫颈上皮内瘤变(CINⅠ-Ⅱ39例,CINⅢ25例)及36例宫颈鳞癌组织的Bag-1和HSP70蛋白进行检测,以20例正常宫颈鳞状上皮作对照。结果:(1)Bag-1在CINⅠ-Ⅱ组、CINⅢ和宫颈鳞癌组的阳性表达率分别为48.72%(19/39)、80.00%(20/25)、83.33%(30/36),宫颈上皮内瘤变及宫颈癌各组阳性率高于正常宫颈鳞状上皮阳性率(P<0.05)。CINⅢ和宫颈鳞癌组的阳性表达率差异无统计学意义(P>0.05)。(2)HSP70在CINⅠ-Ⅱ组、CINⅢ组和宫颈鳞癌组的阳性表达分别为48.72%(19/39)、72.00%(18/25)、86.11%(31/36),各组阳性表达率均高于正常宫颈鳞状上皮(P<0.05)。CINⅢ组和宫颈鳞癌组的阳性表达率差异无统计学意义(P>0.05)。(3)在宫颈鳞癌组织中Bag-1与HSP70之间存在正协同作用(r=0.393,P=0.016)。结论:Bag-1和HSP70在CIN和宫颈鳞癌中的过表达提示它们在宫颈鳞癌的发生、发展起促进作用。Bag-1与HSP70之间存在相关性,说明宫颈鳞癌的发生、发展是多基因调控的过程。
Objective: To investigate the relationship between Bag-1 and heat shock protein 70 (HSP70) in cervical intraepithelial neoplasia. Methods: Immunohistochemical SP method was used to detect the expression of Bag-1 and HSP70 in 64 cases of cervical intraepithelial neoplasia (CINⅠ-Ⅱ39 cases, CINⅢ25 cases) and 36 cases of cervical squamous cell carcinoma. Twenty cases of normal cervical squamous Epithelium as a control. Results: (1) The positive rates of Bag-1 in CINⅠ-Ⅱ group, CINⅢ and cervical squamous cell carcinoma were 48.72% (19/39), 80.00% (20/25) and 83.33% (30/36) The positive rate of cervical intraepithelial neoplasia and cervical cancer in each group was higher than that of normal cervical squamous epithelium (P <0.05). There was no significant difference in the positive expression rates between CINⅢ and cervical squamous cell carcinoma (P> 0.05). (2) The positive expressions of HSP70 in CINⅠ-Ⅱgroup, CINⅢgroup and cervical squamous cell carcinoma group were 48.72% (19/39), 72.00% (18/25) and 86.11% (31/36), respectively Rates were higher than normal cervical squamous epithelium (P <0.05). There was no significant difference in the positive expression rates between CINⅢand cervical squamous cell carcinoma (P> 0.05). (3) There is a positive synergistic effect between Bag-1 and HSP70 in cervical squamous cell carcinoma (r = 0.393, P = 0.016). Conclusion: The overexpression of Bag-1 and HSP70 in CIN and cervical squamous cell carcinoma suggest that they play an important role in the carcinogenesis and progression of cervical squamous cell carcinoma. There is a correlation between Bag-1 and HSP70, indicating the occurrence and development of cervical squamous cell carcinoma is the process of multi-gene regulation.