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利用计算机辅助的精子分析系统(CASA)研究了2,3,7,8—四氯二苯-P-二口恶口英(TCDD)对大鼠精子运动能力的影响。给21天龄幼鼠腹腔注射TCDD0.1、1.0和5.0μg/kg,对照组注射等量体积的溶媒。在性成熟后(90天),用扩散法收集附睾尾精子,测定其运行速度(VCL、VSL、VAP)、运动方式(STR、LIN、BCF、ALH、MAD等)及活动精子的比率(Mot%)。结果表明,TCDD1.0μg/kg组,精子的运动速度明显低于对照组(VCLP<0.05;VAP、VSL、BCFP<0.01),0.5μg/kg时精子的运动速度、及前向性和直线性运动性能均明显降低(P<0.01),活动精子的比率也明显低于对照组(P<0.01)。本研究结果为TCDD的男性生殖毒理学研究,以及CASA在男性生殖毒理学研究中的应用提供了进一步参考依据
The effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on sperm motility in rats were studied using a computer-assisted sperm analysis system (CASA). Twenty (21) days old mice were intraperitoneally injected with TCDD 0.1, 1.0 and 5.0 μg / kg, while the control group was injected with an equal volume of vehicle. After sexual maturity (90 days), spermatozoa of the epididymis were collected by diffusion method and their motility (VCL, VSL, VAP), exercise pattern (STR, LIN, BCF, ALH, MAD, etc.) and motile sperm motility %). The results showed that the sperm motility of TCDD 1.0μg / kg group was significantly lower than that of control group (VCLP <0.05; VAP, VSL, BCFP <0.01), sperm motility at 0.5μg / kg, Both sexual and linear exercise performance were significantly decreased (P <0.01), and the ratio of motile sperm was also significantly lower than that of the control group (P <0.01). The results of this study provide further references for the study of male reproductive toxicology in TCDD and the application of CASA in male reproductive toxicology research