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目的 探讨采用人在位子宫内膜及异位子宫内膜建立子宫内膜异位症动物模型的可行性。方法 获取人分泌晚期在位子宫内膜及人异位子宫内膜种植于NOD -SCID鼠腹部皮下 ,随机分成 4组 ,分别在 2、4、6、8周取出其皮下移植物行病理检查。结果 3 2只小鼠均存活 ,有 15只小鼠皮下在位内膜移植物经HE染色可见明显的子宫内膜腺体和间质 ,成功率为 93 .75 %。而异位内膜移植物仅见到纤维结缔组织。结论 用人在位内膜建立NOD -SCID小鼠子宫内膜异位症模型成功率高 ,模型性状显著且稳定。
Objective To explore the feasibility of using animal endometriosis and ectopic endometrium to establish an animal model of endometriosis. Methods The endometriosis and endometriosis of human endometriosis were planted in the abdomen of NOD-SCID mice subcutaneously and divided into 4 groups at random. The subcutaneous implants were removed at 2,4,6,8 weeks respectively. Results All the 32 mice survived. There were 15 mice with subendothelial ectopic endometrial glands showing obvious endometrial glands and stroma with HE staining. The success rate was 93.75%. Ectopic endometrium graft only see the fibrous connective tissue. Conclusion The establishment of NOD-SCID mice endometriosis model with endometrium has a high success rate, the model traits are significant and stable.