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目的观察白香丹对经前期综合征(PMS)肝气逆证模型大鼠额区和下丘脑中γ-氨基丁酸(GABA)B1受体(GBR1)、γ-氨基丁酸B2受体(GBR2)分布及表达的影响,探讨白香丹对PMS肝气逆证的干预机制。方法通过旷场实验和阴道涂片法筛选健康雌性Wistar大鼠进入实验,采用电刺激法复制PMS肝气逆证大鼠模型,用免疫荧光双标记技术检测GBR1和GBR2在额区和下丘脑中的表达。结果与正常对照组大鼠比较,模型组大鼠垂直得分、水平得分和总分均显著增加(P<0.01),GBR1和GBR2蛋白在额区和下丘脑中的表达水平降低(P<0.01);与模型组大鼠比较,白香丹组和巴氯芬组大鼠旷场得分显著降低(P<0.01,P<0.05),GBR1和GBR2蛋白在额区和下丘脑中的表达水平明显上升(P<0.01,P<0.05),但两组比较差异无统计学意义(P>0.05)。结论 GABABR亚基(GBR1和GBR2)表达的下调可能与PMS肝气逆证的发生密切相关;白香丹可对GBR1、GBR2的表达起到调节作用。
Objective To observe the effect of Bai Xiang Dang on the amount of GABA B1 receptor (GBR1), γ-aminobutyric acid B2 receptor (GBR2) in frontal and hypothalamus of rats with premenstrual syndrome (PMS) ) Distribution and expression of the impact of white Xiangdan PMS liver qi inverse syndrome intervention mechanism. Methods Healthy female Wistar rats were screened by open-field test and vaginal smear method. The rat model of PMS liver-qi invasion was established by electrical stimulation. The expressions of GBR1 and GBR2 in frontal and hypothalamus were detected by immunofluorescence double labeling expression. Results Compared with the normal control group, the vertical score, the level score and the total score of the model group were significantly increased (P <0.01), while the expression of GBR1 and GBR2 protein in the frontal and hypothalamus was decreased (P <0.01) (P <0.01, P <0.05). The expression of GBR1 and GBR2 protein in the frontal and hypothalamus significantly increased compared with the model group (P <0.01, P <0.05), but there was no significant difference between the two groups (P> 0.05). Conclusions The down-regulation of GABABR subunit (GBR1 and GBR2) may be closely related to the occurrence of PMS liver-qi invasion syndrome. Bai Xiang Dan may regulate the expression of GBR1 and GBR2.