论文部分内容阅读
目的 观察中药814对弹性蛋白酶致肺气肿的防治作用.并寻找其作用途径.方法 雄性金黄地鼠90只,分正常对照(生理盐水)组、肺气肿模型组、814用药组,于支气管灌注弹性蛋白酶制作肺气肿模型,于灌注酶前两周给予中药制剂814,4g/100gBW溶于生理盐水灌胃.动物分别于注酶后的一月、两月、三月处死,用显微镜微机测定肺泡平均内村间隔(MLI)、平均肺泡数(MAN)等项指标,观察肺气肿的病变程度.同时作气管肺泡灌洗液中丙二醛含量、血清中α_1-抗胰蛋白酶(α_1-AT))的测定.结果814防治组肺气肿各项指标都表现不同程度的缓解,丙二醛含量在肺气肿组有显著增加.而在814防治组较肺气肿组明显下降;α_1-AT在肺气肿组降低.在814防治组则有所回升.结论814可部分地阻抑弹性蛋白酶地鼠肺气肿的发生发展.从本实验推测814的防治作用与抑制氧自由基的产生、保护或减少α_1-AT丢失有关.
Objective To observe the preventive and therapeutic effects of Chinese medicine 814 on elastase-induced emphysema and to find out the ways of action. Methods 90 male golden hamsters were divided into normal control (saline) group, emphysema model group, and 814 drug group on the bronchus Infuse elastase to make the emphysema model, give Chinese medicine preparation 814 two weeks before infusion of enzyme, 4g/100gBW dissolved in physiological saline gavage. The animals were killed in January, February and March after the injection of enzyme respectively, and the microscope was used. The average alveolar ridge (MLI) and mean alveolar number (MAN) were measured to observe the degree of emphysema. At the same time, the content of malondialdehyde in the bronchoalveolar lavage fluid and the serum α_1-antitrypsin (α_1-) were measured. Measurements of AT)).Results All indexes of emphysema in the 814 prevention and control group showed different degrees of remission, malondialdehyde content increased significantly in the emphysema group, while in the 814 control group the emphysema group was significantly decreased compared to the emphysema group; -AT decreased in the emphysema group. In the 814 control group, there was a rebound. Conclusion 814 may partially suppress the development of elastase-induced hamster emphysema. This experiment speculates 814 on the prevention and treatment of oxygen free radicals. Generate, protect, or reduce loss of α_1-AT .