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目的:探讨干扰素抗病毒应答与慢性乙型肝炎患者外周血树突状细胞(DCs)功能的关系。方法:分别采集23例慢性乙型肝炎患者干扰素治疗前和治疗满4mo时的抗凝外周静脉血,分离外周血单个核细胞(PBMCs),在重组人白细胞介素4和重组人粒细胞-巨噬细胞集落刺激因子的作用下培养7 d使DCs增殖、成熟。以间接免疫荧光流式细胞技术检测DCs的表型;以ELISA法检测DCs单独培养上清液中IL-12的水平;用DCs与HBsAg共同孵育,丝裂霉素C处理后,再与自体PBMCs共同培养,加入H-TDR,收集细胞测定cpm值。实验中以8例正常健康人作为对照。结果:干扰素完全应答组DCs表面CD,HLA-DR和ICAM-1的表达较治疗前均显著增加(均为P<0.05),且CD,CD,HLA-DR和ICAM-1的增高与无应答组相比具有显著性意义(分别为P<0.05、P<0.01、P<0.01和P<0.05);完全应答组DCs分泌IL-12的水平在治疗后显著增加(P<0.01);DCs的抗原提呈作用在治疗前、后,完全应答组比无应答组均显著增强(P<0.01和P<0.001),而部分应答与无应答组之间无显著性差异(P>0.05)。结论:慢性乙肝患者干扰素的抗病毒应答与外周血DCs的功能状态有关,干扰素能显著促进患者DCs功能的改善,进而可能增加患者对治疗的应答。
Objective: To investigate the relationship between interferon antiviral response and peripheral blood dendritic cells (DCs) function in patients with chronic hepatitis B. Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from 23 patients with chronic hepatitis B before anticoagulant therapy and at 4 months after treatment, respectively. Recombinant human interleukin - 4 and recombinant human granulocyte - Under the action of macrophage colony stimulating factor, DCs proliferated and matured for 7 days. The phenotype of DCs was detected by indirect immunofluorescence flow cytometry. The levels of IL-12 in DCs alone culture supernatants were detected by ELISA. After DCs were incubated with HBsAg and mitomycin C, they were incubated with autologous PBMCs Co-culture, add H-TDR, collect cells and measure cpm value. In the experiment, 8 normal healthy people were taken as control. Results: The expressions of CD, HLA-DR and ICAM-1 on the surface of DCs in complete response group of interferon were significantly higher than those before treatment (all P <0.05), and the levels of CD, CD, HLA-DR and ICAM- (P <0.05, P <0.01, P <0.01 and P <0.05, respectively). The levels of IL-12 secreted by DCs in the complete response group were significantly increased after treatment (P <0.01). DCs (P <0.01 and P <0.001). However, there was no significant difference between the partial response and non-response groups (P> 0.05). Conclusion: The antiviral response of interferon in patients with chronic hepatitis B is related to the functional status of peripheral blood DCs. Interferon can significantly improve the function of DCs in patients and may increase the response of patients to treatment.