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目的:探讨感染不同时期内毒素、肿瘤坏死因子(TNF-α)和Gln浓度的变化。方法:将90只大鼠随机分为对照组(正常组)和盲肠结扎穿孔(CLP)的实验组。动物模型制作成功后6、12、24、48和72 h采取血浆、肝、骨骼肌和小肠,并检测血浆内毒素、TNF-α和Gln浓度以及肝、骨骼肌和小肠的Gln浓度。结果:在脓毒症大鼠中,血浆内毒素、TNF-α均于6 h开始升高,12 h显著升高,24 h达高峰,48和72 h显著下降;血浆中Gln 6～12 h升高,24～48 h维持高峰,72 h开始下降;肝内Gln浓度6～12 h升高,24 h显著升高至高峰,48和72 h显著下降,并低于正常水平;小肠、骨骼肌中Gln浓度6～12 h下降不显著,24 h以后显著下降,并低于正常水平。结论:在感染早期(6～12 h),血中内毒素和TNF-α浓度显著升高;血浆和肝内Gln浓度显著升高,小肠和骨骼肌中Gln浓度下降不明显。
Objective: To investigate the changes of endotoxin, tumor necrosis factor (TNF-α) and Gln concentrations in different stages of infection. Methods: 90 rats were randomly divided into control group (normal group) and cecal ligation and puncture (CLP) experimental group. Plasma, liver, skeletal muscle and small intestine were harvested at 6, 12, 24, 48 and 72 hours after the animal model was made. Plasma endotoxin, TNF-α and Gln concentrations, and liver, skeletal muscle and intestinal Gln concentrations were measured. Results: In sepsis rats, plasma endotoxin and TNF-α began to rise at 6 h, significantly increased at 12 h, reached the peak at 24 h and decreased significantly at 48 h and 72 h, and plasma levels of Gln 6-12 h Increased from 24 to 48 h, and then decreased from 72 h. The intrahepatic Gln concentration increased from 6 to 12 h, reached a peak at 24 h, decreased significantly at 48 h and 72 h, and was lower than normal level. Intestinal and skeletal Muscle Gln concentration 6-12 h did not decline significantly, after 24 h significantly decreased, and below normal levels. CONCLUSION: In the early stage of infection (6-12 h), the levels of endotoxin and TNF-α in blood are significantly increased. The concentrations of Gln in plasma and liver are significantly increased, while the concentrations of Gln in small intestine and skeletal muscle are not significantly decreased.