论文部分内容阅读
AIM:To demonstrate that mitochondrial morphological andfunctional changes are an important intermediate link in thecourse of apoptosis in esophageal carcinoma calls inducedby As_2O_3.METHODS:The esophageal carcinoma call line SHEEC1,established in our laboratory,was cultured in 199 growthmedium,supplemented with 100mL·L~(-1)calf serum and3 mol·L~(-1)As_2O_3(the same below).After 2,4,6,12,24 hof drug adding,the SHEECl calls were collected for light-and electron-microscopic examination.The mitochondriawere labeled by Rhodamine fluorescence probe and thefluorescence intensity of the mitochondria was measured byflow cytorneter and cytofluorimetric analysis.Further,themitochondrial transmembrane potential(MTP,ΔΨm)change was also calculated.RESULTS:The mitochondrial morphological change afteradding As_2O_3 could be divided into three stages.In theearly-stage(2-6h)after adding As203,an adaptiveproliferation of mitochondria appeared;in the mid-stage(6-12 h)a degenerative change was observed;and in the late-stage(12-24 h)the mitochondria swelled with outermembrane broken down and then calls death with apoptoticchanges of nucleus.The functional change of themitochondria indicated by fluorescent intensity,whichreflected the MTP status of mitochondria,was in accordancewith morphological change of the mitochondria.Thefluorescent intensity increased at early-stage,decUned inmid-stage and decreased to the lowest in the late-stage.24h after As_2O_3 adding,the cell nucleus showed typicalapoptotic changes.CONCLUSION:Under the inducement of As_2O_3,the earlyapoptotic changes of SHEEC1 cells ware the apparentmorphological and functional changes of mitochondria,afterwards the nucleus changes followed.It is consideredthat changes of mitochondria are an important intermediatelink in the course of apoptosis of esophageal carcinoma cells induced by As_2O_3.
AIM:To demonstrate that mitochondrial morphological andfunctional changes are an important intermediate link in thecourse of apoptosis in esophageal carcinoma callsinducedby As_2O_3.METHODS:The esophageal carcinoma call line SHEEC1,established in our laboratory,was cultured in 199 growthmedium,supplemented with 100mL·L ~(-1)calf serum and3 mol·L~(-1)As_2O_3(the same below).After 2,4,6,12,24 hof drug adding, the SHEECl calls were collected for light-and electron-microscopic examination .The mitochondriawere labeled by Rhodamine fluorescence probe and the fluorescence intensity of the mitochondria was measured by flow cytorneter and cytofluorimetric analysis.Further, the mitochondrial transmembrane potential(MTP,ΔΨm)change was also calculated.RESULTS:The mitochondrial morphological change afteradding As_2O_3 could be divided into three stages.In the early-stage(2-6h)after adding As203,an adaptiveproliferation of mitochondria;;in the mid-stage(6-12 h)a degenerative change was observe d;and in the late-stage(12-24 h)the mitochondria swelled with outermembrane broken down and then calls death with apoptoticchanges of nucleus.The functional change of the mitochondria indicated by fluorescent intensity,whichreflected the MTP status of mitochondria,was in accordance with After morphological change of the mitochondria. Earlyapoptotic changes of SHEEC1 cells ware the apparent morphological and functional changes of mitochondria,afterwards the nucleus changes followed.It is consideredthat changes of mitochondria are an important intermediatelink in the course of apoptosis of esophageal carcinoma cells induced by As_2O_3.