论文部分内容阅读
Although prohibitin 1(PHB) is known to be associated with tumors,there are few studies regarding the role of PHB in hepatocellular carcinoma.An earlier glycoproteomics study of ours showed that PHB is over-expressed in MHCC97-H cells,a highly metastatic liver cancer cell line,and can be captured by wheat germ agglutinin.In the present study,western blotting and reverse transcription-PCR experiments showed an approximately 2-fold up-regulation in the expression of PHB in MHCC97-H cells.However,PHB was not significantly up-regulated in MHCC97-L cells,a low-metastatic liver cancer cell line.When PHB was over-expressed in MHCC97-L cells,cell proliferation was inhibited by 35% and migration increased about 2-fold.The results of this study show that PHB is up-regulated in MHCC97-H cells and is associated with both proliferation and migration of liver cancer cells.
An earlier glycoproteomics study of ours showed that PHB is over-expressed in MHCC97-H cells, a highly metastatic liver cancer cell line, and can be captured by wheat germ agglutinin. In the present study, western blotting and reverse transcription-PCR experiments showed an approximately 2-fold up-regulation in the expression of PHB in MHCC97-H cells. However, PHB was not significantly up-regulated in MHCC97-L cells, a low-metastatic liver cancer cell line. When PHB was over-expressed in MHCC97-L cells, cell proliferation was inhibited by 35% and migration increased about 2-fold.The results of this study show that PHB is up-regulated in MHCC97-H cells and is associated with both proliferation and migration of liver cancer cells.