【摘 要】
：
Excess ammonia (NH3) in the circulation of dairy animals can reduce animal health and the quality of products for human consumption. To develop effective prevention and treatment methods, it is essential to examine the molecular mechanisms through which e
【机 构】
：
College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,P.R.China;Joint Interna
论文部分内容阅读
Excess ammonia (NH3) in the circulation of dairy animals can reduce animal health and the quality of products for human consumption. To develop effective prevention and treatment methods, it is essential to examine the molecular mechanisms through which excess NH3 may affect the mammary gland. The present study used bovine mammary epithelial cells (BMECs) to evaluate the effects of exogenous NH4Cl on the abundance of circular RNAs (circRNAs) using high-throughput sequencing. Among the identified circRNAs, circ02771 was the most significantly upregulated by exogenous NH4Cl (P<0.05), with a fold change of 4.12. The results of the apoptosis and proliferation assays, transmission electron microscopy, H&E staining, and immunohistochemistry revealed that circ02771 increased apoptosis and inflammation. A double luciferase reporter assay revealed that circ02771 targeted miR-194b, and the overexpression of circ02771 (pcDNA-circ02771) reduced (P<0.05) the expression of miR-194b and led to apoptosis and inflammation. Circ02771 also enhanced the expression of transforming growth factor beta-induced factor homeobox 1 (TGIF1), which is a target gene of miR-194b. Overall, this study suggests that the circ02771/miR-194b/TGIF1 axis plays a role in mediating the effects of NH4Cl on BMECs. Therefore, this axis provides a novel target to help control hazards within the mammary gland from high circulating NH4Cl levels.
其他文献
Pakchoi (Brassica campestris L. ssp. chinensis) is an important leafy vegetable. Various light spectra, especially red and blue light, play vital roles in the regulation of nitrate metabolism. Information on the effects of red and blue light on nitrate me
为研究LPCV牛舍夏季舍内环境变化规律,为LPCV牛舍提供合理的湿帘风机运行方案,本文以沈阳地区某六排低屋面横向通风牛舍为研究对象,采用计算流体动力学(Computational fluid dynamics,CFD)方法,分别在沈阳夏季设计工况和极端最热工况下对舍内环境进行三维数值模拟,分析两种工况条件下舍内的温度和速度分布规律,确定两种工况下的湿帘风机运行方案.模拟结果表明,沈阳地区夏季设计工况条件下,风机全开,湿帘喷淋水采用常温水20℃时,舍内环境可满足舍内奶牛舒适性要求;在极端最热工况条件下,风机
The root system architecture (RSA) of a crop has a profound effect on the uptake of nutrients and consequently the potential yield. However, little is known about the genetic basis of RSA and resource adaptive responses in wheat (Triticum aestivum L.). He
Ascorbate peroxidase (APX) plays a key role in scavenging reactive oxygen species (ROS) in higher plants. However, there is very little information available on the APXs in kiwifruit (Actinidia), which is an economically and nutritionally important hortic
脂肪肝是奶牛围产期常见多发的营养代谢性疾病,特别是高产奶牛的发病率极高,常常伴随难产,并诱发多种炎性反应等疾病.此外,还会导致奶牛产奶量减少和平均寿命降低,给奶牛养殖业带来巨大损失.因此探究围产期奶牛脂肪肝的发展进程,对于切实提升高产奶牛的经济价值,和对奶牛脂肪肝的预防与治疗有着重要意义.本文对奶牛围产期脂肪肝的研究情况进行了综述.
Changes in the metabolites of table grapes (Vitis vinifera) reportedly occur during postharvest senescence. The aim of this study was to determine the metabolomic differences in postharvest table grapes (\'Red Globe\') after being subjected to differe
本试验旨在通过对不同来源生鲜乳的相关指标进行测定和比较分析,评价其品质,为地方奶牛养殖业的发展提供参考依据.采集2017-2020年云南省养殖专业合作社、乳品公司养殖场、收购站和机械化挤奶站4种来源的生鲜乳共661个样品,采用国家标准方法测定其相关指标,用SPSS 22.0统计软件做单因素方差分析.结果显示,2017-2020年不同来源生鲜乳中的碱类均为阴性,皮革水解物均未检出;冰点在-0.500～-0.536℃之间,相对密度1.029～1.031g/mL之间,且不同来源间无显著差异(P>0.05).4种
Mechanized grain harvest of maize becomes increasingly important with growing land plot size in Northeast China. Grain moisture is an important factor affecting the performance of mechanized grain harvest. However, it remains unclear what influences grain
Plant parasitic nematodes secrete effector proteins to parasitize hosts successfully. Of these proteins, serine carboxypeptidases have critical roles in pathogenicity. This study investigated the role of new effector AbSCP1 in Aphelenchoides besseyi patho
MicroRNAs (miRNAs) are small (ca. 20–24 nucleotides) non-coding RNAs that have recently been recognized as key post-transcriptional modulators of gene expression; and they are involved in many biological processes in plants, such as root growth and develo