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目的分析海南省2005年霍乱菌株暴发分离株的分子特征及菌株之间的分型相似性,为防治工作提供病原依据,并与PulseNet China提供资料进行相关分析。方法对分离株进行噬菌体-生物分型,通过PCR检测霍乱毒素基因,利用脉冲场凝胶电泳(PFGE)进行分子分型和相似性聚类分析。结果54株分离株中有51株为产毒株,稻叶血清型,噬菌体-生物分型为1b型,其余3株为非流行株。全部菌株被分为15种PFGE带型,聚类分析大部分产毒株之间高度相似,并与PulseNet China提供资料证实,与同时福建、广东、浙江流行的稻叶型菌株带型相同。结论海南省2005年稻叶型暴发菌株,属于2005年流行的菌型,提示在我国东南沿海地区的广泛扩散。
Objective To analyze the molecular characteristics of isolates from outbreaks of cholera strains in Hainan Province in 2005 and their typing similarities among isolates, to provide pathogenic evidence for prevention and treatment and to analyze the data provided by PulseNet China. Methods The phage-typed isolates were isolated and the cholera toxin genes were detected by PCR. The genotypes of cholera toxin were analyzed by pulsed-field gel electrophoresis (PFGE). Results Among the 54 isolates, 51 strains were toxin-producing strains, the rice leaf serotypes and phage-typed strains were type 1b, and the remaining 3 strains were non-epidemic strains. All strains were divided into 15 PFGE bands. Cluster analysis showed that most of the strains were highly similar, and confirmed by the data provided by PulseNet China. The strains were the same as that of the strains of rice leaf type prevailing in Fujian, Guangdong and Zhejiang at the same time. Conclusion In 2005, the outbreak of rice leaf blight in Hainan Province belonged to the endemic type in 2005, suggesting widespread spread in the southeastern coastal areas in China.