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为全面深入了解CRISPR在致病菌中的最新进展,该文简介了CRISPR的结构与功能,深入论述其在各类致病菌中的分型研究及应用情况,剖析CRISPR分型研究现有问题和措施,并展望今后CRISPR重点研究方向。CRISPR分型在各类致病菌中有着不同程度应用,在结核分枝杆菌复合群、沙门氏菌、产志贺毒素大肠杆菌等致病菌中已获成功。加强多种致病菌的全基因组测序及扩充CRISPR数据库,CRISPR分型将会应用更广泛。
In order to provide a comprehensive and in-depth understanding of the recent progress of CRISPR in pathogens, this paper introduces the structure and function of CRISPR, discusses its classification and application in various pathogenic bacteria in detail, and analyzes the existing problems of CRISPR typing And measures, and look forward to the future CRISPR key research directions. CRISPR typing in various types of pathogens have varying degrees of application, in the Mycobacterium tuberculosis complex, Salmonella, Shiga toxin-producing Escherichia coli and other pathogens have been successful. Enhancing genome-wide sequencing of multiple pathogens and expanding the CRISPR database, CRISPR typing will be more widely used.