建立了用RP -HPLC法测定血清中羟基喜树碱含量的方法。文中采用ODS不锈钢柱 (10cm×4.6mm,5μm) ,流动相为甲醇:0.625mol/L磷酸缓冲溶液 (pH6.4)35:65 ,流速为0.5ml/min ,检测波长为385nm ,羟基喜树碱的保留时间为4.4min。回收率为62.0% ,RSD=3.6 % (n=5)。在0～400ng/ml浓度范围内有较好的相关性 ,r=0.9946 ,血清中最低检出限为5ng/m1 A method for the determination of hydroxycamptothecin in serum by RP-HPLC was established. In this paper, ODS stainless steel column (10cm × 4.6mm, 5μm) was used. The mobile phase was methanol: 0.625mol / L phosphoric acid buffer solution (pH6.4) 35:65, the flow rate was 0.5ml / min and the detection wavelength was 385nm. The retention time of the base was 4.4 min. The recovery was 62.0% with RSD = 3.6% (n = 5). In the concentration range of 0 ~ 400ng / ml, there was a good correlation, r = 0.9946, and the lowest detection limit in serum was 5ng / ml