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为了开发能识别正常与错配核酸的探针,首次合成了[Co(phen)2(HNAIP)]Cl3金属配合物,并利用二维核磁研究了此配合物与错配d(CCGAATGAGG)2及正常d(CCTAATTAGG)2寡聚核苷酸的相互作用,首次发现,[Co(phen)2(HNAIP)]Cl3是从“大沟”插入到错配碱基对G3A4和G7A8之间,能够很好地识别G:A错配;而[Co(phen)2(HNAIP)]Cl3与正常的寡聚核苷酸的作用,则是从小沟插入,即[Co(phen)2(HNAIP)]Cl3与错配和正常寡聚核苷酸在不同位置及不同沟槽存在明显的插入式结合、并且有明显的差异,这就有可能被用来作为识别正常与错配寡聚核苷酸的探针.
For the first time, [Co (phen) 2 (HNAIP)] Cl3 metal complexes were synthesized for the first time to develop probes that recognize both normal and mismatched nucleic acids. The complexes were characterized by two-dimensional nuclear magnetic resonance spectroscopy with mismatched d (CCGAATGAGG) 2 and For the first time, we found that [Co (phen) 2 (HNAIP)] Cl3 was inserted from “Ditch ” to the mismatch base pairs G3A4 and G7A8, (Co (phen) 2 (HNAIP)] Cl3 and normal oligonucleotides are inserted into the minor groove, that is, [Co (phen) 2 (HNAIP) ] Cl3 has obvious insertion-type binding to mismatches and normal oligonucleotides at different positions and in different trenches, with significant differences, which may be used to identify normal and mismatched oligonucleotides Probe.