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目的 探讨雌激素 (Estrogen ,E)在 1-甲基 - 4 -苯基吡啶离子 (MPP+ )对PC12细胞损伤过程中对神经细胞的作用机制 ,特别是雌激素与细胞凋亡的关系。方法 用半定量逆转录PCR(RT PCR)检测细胞凋亡过程中促进细胞增殖基因Bcl x和促进细胞死亡基因白细胞介素 1β转换酶 (interleukin 1βconverten zyme ,ICE)的有效蛋白mRNA的表达水平 ,比较各组的差异。 结果 雌激素组的Bcl x的有效蛋白mRNA的表达水平显著高于对照组、MPP+ 组及E +MPP+ 组 (P <0 .0 5 ) ;ICE的有效蛋白mRNA的表达水平显著低于对照组、MPP+ 组及E +MPP+ 组 (P <0 .0 5 )。E +MPP+ 组Bcl x的有效蛋白mRNA的表达水平显著高于MPP+ 组 ;ICE的有效蛋白mRNA的表达水平显著低于MPP+ 组 (P <0 .0 5 )。E +MPP+ 组和对照组相比无显著性差异 (P >0 .0 5 )。结论 雌激素对于MPP+ 诱导的ICE增加和Bcl x降低可起一定的拮抗作用 ,从而在一定程度上抑制了凋亡的发生。
Objective To investigate the mechanism of action of estrogen (E) on neural cells in the injury of PC12 cells induced by 1-methyl-4-phenylpyridinium ion (MPP +), especially the relationship between estrogen and apoptosis. Methods The mRNA expression of Bcl x and ICE in the process of apoptosis were detected by semi-quantitative reverse transcriptase-PCR (RT-PCR). Differences in each group. Results The mRNA expression of Bcl-x in the estrogen group was significantly higher than that in the control group, MPP + group and E + MPP + group (P <0.05). The mRNA expression of ICE was significantly lower than that of the control group MPP + group and E + MPP + group (P <0.05). The mRNA expression of Bclx in E + MPP + group was significantly higher than that in MPP + group. The mRNA expression of ICE was significantly lower than that in MPP + group (P <0.05). There was no significant difference between E + MPP + group and control group (P> 0.05). Conclusion Estrogen can antagonize the increase of ICE induced by MPP + and the decrease of Bcl x, thus inhibiting the apoptosis to a certain extent.