Objective:To explore the effects of curcumin on invasion and metastasis in the human cervical cancer cells Caski.Methods:Caski cells were treated with 10,25,50μmol/L curcumin for 24,48,72 h.Proliferation of Caski cells was measured with MTT assay.When treated with 50μmol/L curcumin for 72 h,the expressions of MMP-2,MT1-MMP and NF-κB of cells were detected by Western-blot,and invasion and metastasis of Caski cells were evaluated with transwell chamber.Results:After being treated with 10μmol/L,25μmol/L,50μmol/L curcumin for 24,48 and 72 h,the proliferation of Caski cells was inhibited in a dose-and time-dependent manner.The expression of MMP-2,MT1-MMP and NF-κB were decreased when being treated with 50μmol/L curcumin for 72 h.After treatment with 50μmol/L curcumin,in invasion assay,the number of cells in curcumin treated group to migrate to filter coated with Matrigel was reduced compared with control group(P<0.05).Meanwhile,in migration assay,the number of cells in curcumin treated group to migrate to filter was also decreased compared with control group(P<0.05).Conclusion:Curcumin could affect the invasion and metastasis of the human cervical cancer cells Caski.Inhibiting the expression of MMP-2,MT1-MMP and NF-κB was probably one of its molecular mechanisms. Objective: To explore the effects of curcumin on invasion and metastasis in the human cervical cancer cells Caski. Methods: Caski cells were treated with 10, 25, 50 μmol / L curcumin for 24, 48, 72 h. Proliferation of Caski cells was measured with MTT assay. Treatment with 50 μmol / L curcumin for 72 h, the expressions of MMP-2, MT1-MMP and NF-κB of cells were detected by Western-blot, and invasion and metastasis of Caski cells were evaluated as transwell chamber. Results: After being treated with 10 μmol / L, 25 μmol / L, 50 μmol / L curcumin for 24, 48 and 72 h, the proliferation of Caski cells was inhibited in a dose- and time-dependent manner.The expression of MMP- MT1-MMP and NF-κB were decreased when being treated with 50 μmol / L curcumin for 72 h. After treatment with 50 μmol / L curcumin, in invasion assay, the number of cells in curcumin treated group to migrate to filter coated with Matrigel was reduced compared with control group (P <0.05). Meanwhile, in migration assay, the number of cells in curcumin tre ated group to migrate to filter was also decreased compared with control group (P <0.05) .Conclusion: Curcumin could affect the invasion and metastasis of the human cervical cancer cells Caski. Inhibit the expression of MMP-2, MT1-MMP and NF- κB was probably one of its molecular mechanisms.