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以小麦(Triticum aestivum L.)双端体3DL为细胞学标记,用具有phKL基因的小麦地方品种“开县罗汉麦”为受体连续回交,将促进小麦外源部分同源染色体配对的phKL基因和Ph2基因缺失重组在一起获得了重组体phKL+Ph2-。这种重组体有正常的育性。与只有phKL基因的小麦材料相比,重组体与外源物种Aegilopsvariabilis Eig.或黑麦(Secale cereale L.)杂种的部分同源染色体配对水平显著增加,表明Ph2基因的缺失体与phKL基因可能存在加性效应。部分同源染色体配对水平的增加表现在棒状二价体、环状二价体和三价体的数量变多而单价体数量减少。单价体的减少主要是由于棒状二价体的增加所造成的。在小麦外源遗传转移中,运用重组体phKL+Ph2-可能比单纯应用Ph2缺失或phKL基因材料更理想。当与具有ph1b基因的材料比较时发现,重组体phKL+Ph2-与Ae. variabilis(或黑麦)杂种的部分同源染色体配对水平显著降低,这主要是由环状二价体和多价体的减少造成的,但是棒状二价体数量表现增加(与Ae. variabilis杂种)或达到类似水平(与黑麦杂种),这一有趣的发现从表现型上证明了Ph1基因与Ph2或phKL基因在诱导部分同源染色体配对时的遗传作用机制存在差异。
Using the 3DL of Triticum aestivum L. as a cytological marker, the phKL gene, a wheat local cultivar with phKL gene, was successively backcrossed with the receptor of “Kaixian Lohan Mai” and the phKL gene Recombinant phKL + Ph2- was obtained by recombination with Ph2 deletion. This recombinants have normal fertility. The homologous chromosome pairing level of the recombinant with the exogenous species Aegilopsvariabilis Eig. Or Secale cereale L. hybrids significantly increased compared to the wheat material only with the phKL gene, suggesting that deletions of the Ph2 gene and the phKL gene may be present Additive effect. Partially homologous chromosome pairing level increases in the rod-shaped divalent body, the number of cyclic divalent and trivalent body increased and the number of monovalent bodies decreased. The decrease of monovalent is mainly due to the increase of rod-shaped divalent body. In exogenous genetic transfer of wheat, the use of recombinant phKL + Ph2- may be more desirable than the use of Ph2 deletion or phKL gene alone. When compared to the material with the phIb gene, it was found that the homologous chromosome pairing level of the recombinant phKL + Ph2- with the Ae. Variabilis (or rye) hybrid was significantly reduced mainly by the cyclic divalent and multivalent , But an increase in the number of rod-shaped bivalents (with Ae. Variabilis hybrids) or at similar levels (with rye hybrids), this interesting finding proves phenotypically to be a significant association between the Ph1 gene and the Ph2 or phKL genes There are some differences in the mechanism of genetic action when some homologous chromosomes are induced.